碘致NOD.H-2^(h4)小鼠自身免疫甲状腺炎的实验研究  

Experimental investigation on iodine-induced autoimmune thyroiditis in NOD.H-2^(h4) mice

作　　者：滕晓春[1] 李静[1] 单忠艳[1] 范晨玲[1] 王红[1] 郭锐[1] 滕卫平[1] 

机构地区：[1]中国医科大学附属第一医院内分泌科,中国医科大学内分泌研究所,沈阳110001

出　　处：《中华内分泌代谢杂志》2007年第6期501-505,共5页Chinese Journal of Endocrinology and Metabolism

基　　金：国家自然科学基金(30370680);辽宁省科技攻关计划(2003225005)

摘　　要：目的探讨碘致自身免疫甲状腺炎发生过程中炎性细胞浸润和甲状腺自身抗体的动态变化。方法NOD.H-2^(h4)和昆明种小鼠各128只随机分入普通水组和0.05%碘化钠水组,分组后1、2、4、8、12、16、24周小鼠眶静脉取血,测定血清自身抗体和甲状腺激素,甲状腺组织制作石蜡切片,HE染色。结果NOD.H-2^(h4)小鼠饮用0.05%碘化钠水1周即有自身免疫性甲状腺炎的发生,补碘12周时患病率和炎性细胞浸润程度达最高峰。补碘8周,其血清TgAb开始升高,之后随着补碘时间的延长,其水平逐渐增高,而对照组血清TgAb始终保持低平曲线。结论碘过量可以促进NOD.H-2^(h4)小鼠自身免疫甲状腺炎(甲状腺淋巴细胞浸润)的发生和发展,血清TgAb是其炎症反应的一个标志。Objective To investigate the kinetic changes of inflammatory cell infiltration and thyroid autoantibodies in the development of iodine-induced autoimmune thyroiditis in NOD. H-2^h4 mice. Methods Either 128 five-week-old NOD. H-2^h4 mice or 128 Kunming mice were randomly divided into two groups, and received plain water or water containing 0. 05% sodium iodide. At the time points of 1,2, 4, 8, 12, 16, and 24 week after receiving iodinated water, mice were anesthetized by diethyl ether and bled from eye socket vein, and their thyroid glands were collected. Indirect ELISA method was used to measure the levels of serum thyrog!obulin autoantibodies （TgAb） and thyroid hormone. After being fixed with paraformaldehyde and embedded in paraffin, thyroid sections were stained with HE and used for morphometrical analysis. Results In the iodine treated group of NOD. H-2^h4 mice, autoimmune thyroiditis was observed as early as 1 week after they began receiving indinated water. The prevalence as well as the degree of autoimmune thyroiditis reached the maximum at 12 weeks and remained until 24 weeks. Serum TgAb level increased after 8 weeks of iodine ingestion in NOD. H-2^h4 mice, then increased steadily throughout the 24 weeks of experiment. On the contrary, serum TgAb was not increased in the control group of Kunming mice. Conclusion Iodine may induce and exacerbate lymphocytic infiltration of the thyroid in genetically susceptible NOD. H-2^h4 mice, and serum TgAb is just a marker of autoimmune thyroiditis.

关 键 词：碘 NOD.H-2^h4小鼠 甲状腺炎 自身免疫性 抗体 甲状腺球蛋白 

分 类 号：R581.4[医药卫生—内分泌] R-332[医药卫生—内科学]