益气活血法对脑出血大鼠脑内血管内皮生长因子蛋白表达的影响  

Effects of Yiqi Huoxue therapy on the expression of VEGF in intracerebral hemorrhagic rat brains

作　　者：刘宜峰[1] 邢之华[1] 唐涛[1] 阳鹤鹏[1] 陈柏林[1] 

机构地区：[1]中南大学湘雅医院中西医结合研究所,湖南长沙410008

出　　处：《中国老年学杂志》2008年第12期1064-1066,共3页Chinese Journal of Gerontology

基　　金：中国博士后科学基金(2005038224);湖南省博士后专项资助计划(2006FJ4244);湖南省青年骨干教师培养对象经费资助项目(湘教通〔2005〕247号);湖南省自然科学基金(07JJ5007)

摘　　要：目的观察脑出血大鼠脑组织中血管内皮生长因子(VEGF)表达,探讨益气活血法治疗脑出血的分子生物学机制。方法Ⅶ型胶原酶诱导大鼠脑出血模型,分别以补阳还五汤及该方的拆方益气方和活血方灌胃,Western印迹法观察出血后1、4、7、14、21、28d鼠脑VEGF蛋白表达。结果模型组、益气组、活血组和益气活血组造模后1d均可检测到VEGF蛋白阳性信号,益气活血组于14d达高峰(P<0.01),早于其他3组(21d);益气活血组第7～28天均高于模型组、益气组、活血组水平(P<0.01)。结论脑出血大鼠脑VEGF蛋白表达上调;益气活血法能增强脑出血大鼠脑VEGF蛋白表达,促进VEGF峰值提前,并维持在较高水平,以促进脑出血损伤修复。Objective To investigate the effect of Yiqi Huoxue therapy on VEGF expression in rat brains after intraeerebral hemorrhage （ICH） for exploring molecular biology therapeutic mechanism of ICH. Methods ICH model was made by injecting eollagenase type Ⅶ stereotaxieally into right globus. Yiqi therapy, Huoxue therapy and Yiqi Huoxue therapy groups were orally administered with Yiqi decoction, Huoxue decoction and Buyang Huanwu decoction respectively to examine the expression of VEGF in rat brains at different time points （ 1,4, 7, 14, 21, 28 d） by Western blot. Results From the 1 st day to 28th day, the expression of VEGF could be observed in the four groups besides the sham group. The level of VEGF achieved peak in Yiqi Huoxue therapy group, was earlier than that in the other three groups（ P 〈0. 01 ）, and the level was higher than other three groups from 7th day to 28th day （ P 〈0. 01 ）. Conclusions The expression of VEGF is up-regulated in ICH rats＇ brain. Yiqi Huoxue therapy could enhance this expression, promote the peak value of VEGF to arrive earlier and delay its reduction to repairing cerebral injury.

关 键 词：益气活血法 脑出血 血管内皮生长因子 

分 类 号：R277.743[医药卫生—中医学]