运动对大鼠海马长时程增强效应及其相关因子的影响  

Effect of Physical Exercise on Hippocampal Long-term Potentiation and Related Factor in Rats

作　　者：马强[1] 陈学伟[1] 王静[1] 安改红[1] 刘洪涛[1] 

机构地区：[1]军事医学科学院卫生学环境医学研究所,天津300050

出　　处：《中国运动医学杂志》2008年第4期451-453,共3页Chinese Journal of Sports Medicine

基　　金：国家自然科学基金面上项目(No30570895)资助

摘　　要：目的:探讨运动对大鼠海马长时程增强(long-term potentiation,LTP)效应及其相关因子的影响。方法:28只成年雄性Wistar大鼠,随机分为对照组与运动组,每组14只。运动组采用4周自愿跑轮运动为体力运动模型。脑立体定位-神经电生理法检测海马齿状回LTP;高效液相-电化学法测定海马5-羟色胺(5-HT)含量;实时荧光定量PCR测定海马5-HT1A受体、环磷酸腺苷反应元件结合蛋白(CREB)和脑源性神经营养因子(BDNF)mRNA表达。结果:运动组海马齿状回LTP较对照组明显提高(P<0.05),运动组海马5-HT含量(P<0.01)、5-HT1A受体mRNA(P<0.01)、CREB和BDNF mRNA表达水平也显著高于对照组(P<0.05)。结论:运动可能通过对5-HT—5-HT1A受体—cAMP—CREB—BDNF—LTP通路元件的上调机制,发挥增强海马LTP的作用。Objective To study the mechanisms underlying physical exercise produced long-term potentiation （LTP） enhancement in hippocampus of rats. Methods Twenty Eight adult male Wistar rats were randomly divided into control group （sedentary group） and exercise group （4-week voluntary wheel running）. LTP in hippocampal dentate gyrus （DG） was recorded by stereotaxic neuronal electrophysiological methods. Levels of hippocampal 5-HT were detected by high-performance liquid chromatography using electrochemical detection. The mRNAs for 5-HT1A receptor, cAMP-response-element binding protein （CREB） and brain-derived neurotrophic factor （BDNF） were measured by real-time fluorescence quantitative reverse transcription polymerase reaction （RT-PCR）. Results Compared with the sedentary controls, enhanced hippocampal LTP （P（0. 05）, increased hippocampal 5-HT levels （P〈0. 01）, up-expressed mRNA levels for 5-HT1A receptor （P〈0. 01）, CREB and BDNF （P〈0. 05） in rats were found in 4 weeks physical exercise group. Conclusion 5-HT, 5-HT1A receptor, CREB and BDNF were likely act as important modulators to be involved in exercise-induced LTP promotion process, in which up-regula- tion of the related factors in 5-HT--5-HT1A receptor--cAMP--CREB--BDNF-LTP pathway might take action.

关 键 词：运动 长时程增强(LTP) 5-羟色胺 环磷酸腺苷反应元件结合蛋白 脑源性神经营养因子 

分 类 号：R87[医药卫生—运动医学]