培养基和继代时间对番茄叶片愈伤组织诱导和芽分化的影响  

Effect of Mediums and subculture time on callns induction and bnd differentiation of tomato leaf

作　　者：毕建水 李翠翠[2] 徐丽丽[3] 

机构地区：[1]莱阳市农业局,山东莱阳265200 [2]青岛农业大学生命科学院,山东青岛266109 [3]青岛农业大学植物保护学院,山东青岛266109

出　　处：《安徽农学通报》2008年第13期41-42,124,共3页Anhui Agricultural Science Bulletin

摘　　要：对两种番茄品种樱红和J07进行了筛选最佳培养基和培养条件的研究结果表明:樱红的叶片外植体在附加激素6-BA 1.0mg/L+IAA 0.1mg/L的改良MS培养基中诱导直接分化芽的能力最强,继代时间为12d时褐化最轻;而最适合J07叶片外植体分化的培养基为附加激素6-BA 1.0mg/L+IAA 0.2mg/L的改良MS培养基,最佳继代时间为20d。The optimal mediums and subculture time of two tomatoes species （yinghong, J07）for tissue culture were stndied in this article. The result indicated that the explants of yinghong leaf blade were induced to buds directly stronger in modified MS medium adding 1.0mg/L 6 - BA and 0. lmg/L IAA than other mediums and browning was lighter taking 12d as subculture time; the optimal medium for explants differentiation of J07 leaf blade was modified MS medium adding 1.0mg/L 6 - BA and 0. 2mg/L IAA and optimal subculture time was 20d.

关 键 词：番茄 组织培养 继代 褐化 

分 类 号：S641.12[农业科学—蔬菜学]