乙型肝炎病毒感染上调2.2.15细胞系程序性死亡分子配体表达的研究  

HBV infection upregulates the PD-L expression on HepG 2.2.15 cell

作　　者：何豫[1] 于贤杰[1] 陈姬[1] 王贵强[1] 

机构地区：[1]北京大学第一医院感染疾病科,北京100034

出　　处：《中国实用内科杂志》2008年第7期540-541,545,共3页Chinese Journal of Practical Internal Medicine

基　　金：卫生部临床学科重点项目(20010911);北京大学985青年基金

摘　　要：目的研究程序性死亡分子配体在HepG2和2.2.15细胞表面的表达及其与HBV感染的关系。方法2007年3月至7月于北京大学第一医院分别培养HepG2细胞和2.2.15细胞,用荧光素标记的抗程序性细胞死亡分子配体-1、2(PD-L1、PD-L2)特异性抗体标记,行流式细胞仪检测;同时提取细胞核糖核酸(RNA),用逆转录聚合酶链反应(RT-PCR)检测PD-L1和PD-L2mRNA的表达。结果PD-L1在2.2.15细胞的表达显著上调;无论是膜蛋白质或是mRNA形式,PD-L2在HepG2细胞和2.2.15细胞均不表达。结论HBV感染导致肝细胞表面程序性死亡分子的配体表达上调,提示与病毒免疫逃避有关。Objective To investigate the expression of programmed cell death-1 （PD-1）ligands on HepG2 cell and 2. 2. 15 cell. Methods Culture HepG2 cell and 2. 2. 15 cell in vitro and stain the cells with fluorescence-labeled monoclonal antibodies specifically against PD-L1 and PD-L2 ,respectively. Isolate total RNA of HepG2 cell and 2. 2. 15 cell and detect the mRNA expressions of PD-L1 and PD-L2 by RT-PCR. Results The expression of PD-L1 on 2.2. 15 cell was upregulated on the levels of both proteins and mRNA. The expression of PD-I2 in 2. 2. 15 cell was not detected. And neither the PD-L1 nor PD-L2 was detected in HepG2 cell. Conclusion HBV infection can upregulate the expression of PD- L1 on hepatocyte,which might contribute to the immune evasion of HBV.

关 键 词：乙型肝炎病毒 程序性死亡分子-1 程序性死亡分子配体1/2 肝细胞 

分 类 号：R5[医药卫生—内科学]