基于高灵敏探针海萤荧光素类似物的化学发光法检测尿微量白蛋白  

Chemiluminescence Method for the Determination of Human Serum Albumin by Highly Sensitive Chemiluminescence Probe-Fluoresceinyl Cypridina Luciferin Analog

作　　者：徐未[1] 魏言春[1] 邢达[1] 陈群[1] 

机构地区：[1]华南师范大学激光生命科学研究所暨激光生命科学教育部重点实验室,广州510631

出　　处：《分析化学》2008年第1期57-60,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.30470494;30627003;60678050);广东省自然科学基金(No.015012)资助项目

摘　　要：海萤荧光素类似物FCLA能检测1O2并产生532nm的化学发光,白蛋白(HSA)能强烈增强FCLA-1O2化学发光。基于此,建立了一种有效、高灵敏的检测HSA的化学发光(CL)检测技术。在选定的最佳实验条件下,HSA浓度与增强化学发光信号在0.34～5.5mg/L之间呈良好的线性关系;检出限为0.017mg/L(3σ)。对比荧光光谱分析法、同步荧光扫描法及瑞利光散射法等传统方法,本方法成本低廉、灵敏度高。实际尿样检测与临床检测结果一致,同时还初步探讨了HSA增敏CL的机制。Highly sensitive chemiluminescence（CL） enhancing method was developed to detect human serum albumin（HSA） based on the reaction of fluoresceinyl cypridina luciferin analog （FCLA）-^1O2. Experiments revealed that chemiluminescence could be greatly enhanced by the addition of HSA. Owing to the enhancing effect of HSA, under the optimal conditions, the enhanced CL intensity was linear for a HSA concentration of 5×10^-9 -8×10^-8 mol/L, with a detection limit of 0. 017 mg/L. The method has a low cost and a higher sensitivity than thase of fluorescence spectra analysis, synchrony fluorescence scanning technique, rayleigh light scattering technique. Determination results for HSA in urine were consistent with clinical ones. Additionally, the mechanism of enhanced CL by HSA was discussed.

关 键 词：化学发光 白蛋白 海萤荧光素类似物 单态氧 

分 类 号：R446.1[医药卫生—诊断学]