激光诱导荧光检测微流控芯片生化分析仪的研制  

Development of Integrated Microfluidic Chip-based Bioanalyzer with Laser-induced Fluorescence Detection

作　　者：程永强[1] 张涛[1] 王鹗 王伟 徐光明[1] 方群[1] 

机构地区：[1]浙江大学化学系微分析系统研究所,杭州310058 [2]上海光谱仪器有限公司,上海200233

出　　处：《分析化学》2008年第1期127-131,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.20575059);教育部(No.NCET-05-0511);浙江省科技厅(No.2004C13001);上海光谱有限公司资助

摘　　要：研制出一种集激光诱导荧光检测、微流控芯片电泳及控制系统于一体的生化分析仪。分析仪内采用可重复使用的玻璃基质微流控芯片,利用销式固定技术实现芯片的精确定位,定位精度达到±2μm。以四触点高电压系统控制芯片上的进样和电泳分离操作。激光诱导荧光检测系统采用正交光路模式,对Cy5染料的检出限达到1.0×10-10mol/L(S/N=3)。以羟乙基纤维素为筛分介质,初步进行了ΦΧ174-HaeШdigest DNAmarker限制性片段的毛细管电泳分离。An integrated bioanalyzer involving microfluidic chip, high voltage power supply, laser-induced fluorescence （LIF） detection system and control system was developed to perform capillary electrophoresis separation. An orthogonal optical arrangement was employed in the LIF detection system to simplify the structure of the system with a limit of detection of 1.0×10^-10 mol/L for Cy5 dye. A simple and convenient approach for accurately locating the chip in the bioanalyzer was developed by using a chip supporter and base pegs with a chip position variation of ± 2 μm. The performance of the present bioanalyzer was demonstrated in the CE separation of a ФX174-HaeⅢ digest DNA marker.

关 键 词：微流控芯片 毛细管电泳 生化分析仪 激光诱导荧光检测 

分 类 号：O657.3[理学—分析化学]