酶荧光毛细分析法测定葡萄糖  

Determination of Glucose by Enzyme Fluorescence Capillary Analytical Method

作　　者：杜运动[1] 高秀峰[2] 李永生[1] 

机构地区：[1]四川大学化学工程学院,成都610065 [2]四川大学华西基础医学与法医学院,成都610041

出　　处：《分析化学》2008年第7期991-994,共4页Chinese Journal of Analytical Chemistry

基　　金：四川大学214人才引进振光计划基金(No0082204127067)资助项目

摘　　要：基于酶催化和荧光毛细分析法(FCA)开发了一种微量、快速测定葡萄糖的新方法(GE-FCA)。优选的实验条件为:反应时间15min;反应温度30℃;磷酸盐缓冲液(pH6.0),HRP浓度和GOD浓度分别是200和150U/L;线性范围0.1～4mg/L;检出限0.070mg/L。GE-FCA对血液中葡萄糖进行测定,其回收率在98.9%～103.8%之间。与其它方法相比,GE-FCA法操作简单,而且试样用量少(仅为18μL),节省了酶试剂用量,实验成本低,易于普及推广。A method was developed for the quick determination of glucose using fluorescence capillary analysis（FCA）.The experimental conditions were as follows：reaction time,15 min;temperature：30 ℃;pH of phosphate buffer solution：6.0;the concentrations of herseradish peroxidase（HRP） and glucosse oxidase（GOD） were 200 U/L and 150 U/L,respectively.The linear range of FCA was 0.1-4 mg/L,the relative standard deviation（RSD） was 〈1.2%,and the detection limit of glucose was 0.07 mg/L.The method has been applied for the determination of glucose in blood,the recovery was 98.9%-103.8%.Compared with the other methods,the FCA had a more simple operation.A tiny sample volume was required（only 18 μL）,so it has the advantage for saving the enzyme reagent.

关 键 词：荧光毛细分析法 血液 葡萄糖 葡萄糖氧化酶 辣根酶 

分 类 号：R587.1[医药卫生—内分泌] R446.1[医药卫生—内科学]