中华卤虫无节幼体蛋白质组的双向凝胶电泳分析  

Two-dimensional gel electrophoresis analysis of the proteome in the larvae of bring shrimp,Artemia sinica

作　　者：周茜[1,2] 吴长功[1] 刘凤岐[3] 黄冰心[1] 相建海[1] 

机构地区：[1]中国科学院海洋研究所,山东青岛266071 [2]中国科学院研究生院,北京100049 [3]南开大学生命学院,天津300071

出　　处：《海洋科学》2008年第7期62-64,共3页Marine Sciences

基　　金：国家重点基础研究发展规划项目(20060109Z4016)

摘　　要：应用双向凝胶电泳和图像分析手段,对比了卤虫(Artemia)初孵无节幼体和孵化24 h无节幼体中蛋白表达的变化情况。结果显示,随着卤虫幼体的发育,蛋白表达的种类和个数均明显增多,仅有个别蛋白的表达量下调。这些差异表达的蛋白可能跟卤虫幼体的发育过程密切相关或起重要的调节作用。Brine shrimp （Artemia） is a worldwide-distributed crustacean, widely used as a main food resource in aquaculture and applied in basic research areas ranging from developmental biology to evolution and ecology. In the present study, using two-dimensional gel electrophoresis and image analysis system,the changes of protein expression in new hatched and 24 h post-hatching Arternia larvae were investigated. Overall, the variety and amount of the proteins increase significantly in accompany with the larvae development. However, the amounts of some proteins were down-regulated. The results suggested that these differentially expressed proteins may tightly associated with, or play important regulative roles in the development of brine shrimp larvae.

关 键 词：蛋白质组 中华卤虫(Artemia sinica) 幼体 双向电泳 

分 类 号：Q51[生物学—生物化学]