量子点标记免疫凝集分析检测免疫球蛋白  

An Aggregation-Based Immunoassay for Detecting Immunoglobulin Using Quantum Dots

作　　者：邵君[1] 高峰[1] 尤晓刚[1] 贺蓉[1] 崔大祥[1] 

机构地区：[1]上海交通大学微纳科学技术研究院,微米/纳米加工技术国家重点实验室,薄膜与微细技术教育部重点实验室,上海200240

出　　处：《上海交通大学学报》2008年第5期827-830,共4页Journal of Shanghai Jiaotong University

基　　金：国家自然科学基金(20771075);浦江人才计划(06PJ14049);上海市科委纳米专项基金(0752nm024)资助项目

摘　　要：利用量子点特殊的光学性能,结合免疫分析技术,建立快速、简便的免疫凝集分析检测方法.采用量子点标记羊抗鼠IgG,与溶液中的鼠IgG进行免疫反应,使量子点之间凝集形成网络结构,从而引起反应体系中量子点的荧光淬灭.随着鼠IgG量的增加,网络结构越来越大,量子点的荧光强度不断减弱.根据荧光信号的变化,检测溶液中鼠IgG的浓度.A rapid and simple method of detection immunoglobulin was developed by using quantum dots labeled immunoassay. The Goat anti-Mouse IgG labelled quantum dots are mixed with Mouse IgG. After the immunoreaction, the quantum dots are aggregated to form a network, which can quench the fluorescence of themselves in the reaction system. The more Mouse IgG is, the bigger the network is, and the fluorescence intensity of quantum dots decreases continually. The concentration of Mouse IgG can be detected by the measurement of relative fluorescence intensity.

关 键 词：量子点 免疫分析 荧光淬灭 鼠IgG 

分 类 号：R446.61[医药卫生—诊断学]