蜻科部分种类Cytb基因序列比较及系统发育分析(蜻蜓目:差翅亚目)  

Comparison of Cytochrome b Gene Sequences and Phylogenetic Analysis of Partial Libellulidae (Odonata: Anisoptera)

作　　者：张大治[1] 杨国辉[2] 郑哲民[3] 

机构地区：[1]宁夏大学生命科学学院,宁夏银川750021 [2]大理学院生命科学与化学学院,云南大理671000 [3]陕西师范大学动物研究所,陕西西安710062

出　　处：《宁夏大学学报（自然科学版）》2008年第2期161-165,共5页Journal of Ningxia University(Natural Science Edition)

基　　金：宁夏自然科学基金项目资助(NZ0707);宁夏大学自然科学基金项目资助(NS0511)

摘　　要：从分子生物学角度入手,以mtDNA的Cytb(Cytochromeb)基因作为分子遗传标记,用昆虫Cytb基因的特异性引物进行PCR扩增及DNA测序,共获得蜻蜓目差翅亚目蜻科7属8种(基斑蜻Libellula depressa,黄蜻Pantala flavescens,庆褐蜻Trithemis festive,晓褐蜻Trithemis aurora,纹蓝小蜻Diplacodes trivialis,网脉蜻Neurothemis fulvia,六斑曲缘蜻Palpoleura sex-maculata,玉带蜻Pseudothemis zonata)及2种外群(黄翅绿色蟌Mnais auripennis,华艳色蟌Neurobasischinensis)的Cytb基因部分序列(576 bp).这8种蜻蜓Cytb基因的部分核苷酸序列(长度为576 bp)中碱基T,C,A,G的平均含量分别为37.0%,18.3%,31.3%和13.4%,A+T平均含量为68.3%,明显高于G+C平均含量(31.7%).密码子第3位点的A+T平均含量较高,为81.0%,碱基替换多发生在密码子第3位点.以束翅亚目的黄翅绿色蟌和华艳色蟌作为外群构建系统发育树,结果显示玉带蜻属与褐蜻属、脉蜻属与曲缘蜻属的亲缘关系较近.Based on 576 bp sequences of Cytb gene by MEGA 2.0 biosoftware, the nucleotide composition and the phylogenetic relationship among 8 species （Libellula depressa, Pantala flavescens, Trithemis festive, Trithemis aurora, Diplacodes trivialis , Neurothemis fulvia , Palpoleura sex-maculata , Pseudothemis zonata） in Libellulidae were dealt with. The results showed that the average contents of T, C, A and G are 37.0%, 18.3%, 31.3% and 13.4%. The contents of A＋T （68.3 %） is obviously higher than that of G＋C （31.7 %）, and come up to 81.0 % at the third position of cordon. The replacement of nucleotides mostly occurs at the third position. Employing Mnais auripennis and Neurobasis chinensis as outgroups, the phylogenetic trees shows that the relationship between Pseudothemis and Trithemis are more closed than that other genera. Neurothemis and Palpoleura are the sister group of Diplacodes, Libellula and Pantala.

关 键 词：CYTB 线粒体DNA 系统发育 蜻科 蜻蜓目 

分 类 号：Q969.22[生物学—昆虫学]