抑制性消减杂交(SSH)技术及其应用  

Suppression Subtractive Hybridization (SSH) and Its Application

作　　者：刘静[1] 赵颖[1] 

机构地区：[1]华北制药集团新药研究开发有限责任公司,河北石家庄050015

出　　处：《河北化工》2008年第7期31-32,37,共3页Hebei Chemical Industry

摘　　要：抑制性消减杂交技术(SSH)是一种高效检测差异表达基因的方法。详细论述了抑制性消减杂交的基本原理及过程,并简要介绍了其在工业生产菌种改良中的应用。Suppression subtractive hybridization （SSH） is an effective approach to identify the genes that vary in expression levels. We have described the basic theory and procedure of SSH in detail. A brief introduction of the application of SSH to the industrial strains improvement has also been given in this paper.

关 键 词：抑制性消减杂交 SSH 差异表达 消减文库 

分 类 号：Q786[生物学—分子生物学]