云南非洲菊根腐病病原鉴定  

Identification of the Pathogens Causing Root Rot of Gerbera jamesonii in Yunnan

作　　者：李越[1] 李凡[1] 刘云龙[1] 李玲[1] 陈精兰[1] 唐小艳[1] 陈海如[1] 

机构地区：[1]教育部农业生物多样性与病虫害控制重点实验室,云南农业大学植物保护学院,云南昆明650201

出　　处：《华南农业大学学报》2008年第3期21-24,28,共5页Journal of South China Agricultural University

基　　金：云南省自然科学基金重点项目(2004C0007Z)

摘　　要：从云南非洲菊主产区表现根腐症状的非洲菊病株上分离到13个镰刀菌菌株及18个疫霉菌菌株,经形态学鉴定、鉴别寄主测定及分子鉴定,分别确定为茄病镰刀菌Fusarium solani(Mart.)Sacc.和隐地疫霉Phytophthora cryp-togea Pethybridge&Lafferty.将茄病镰刀菌和隐地疫霉回接到健康非洲菊根部,隐地疫霉可以单独引起根腐,而茄病镰刀菌不能单独使植株表现明显发病症状.将茄病镰刀菌和隐地疫霉混合接种后,发现非洲菊根腐症状加重,且发病时间缩短.表明隐地疫霉是引起云南非洲菊根腐病的主要病原,茄病镰刀菌对非洲菊根腐病的发生起到加速发病时间和加重发病症状的作用.Thirteen isolates of Fusarium solani and eighteen isolates of Phytophthora cryptogea were isolated from the diseased Gerbera jamesonii Bolus showing symptom of root rot in Yunnan Province. The isolates of F. solani was identified as F. solani（ Mart. ）Sacc. and the isolates of P. cryptogea was identified as P. cryptogea Pethybridge ＆ Lafferty by morphology, by the methods of differential hosts test and molecular identification. F. solani and P. cryptogea were re-inoculated to healthy G. jamesonii. It was showed that only P. cryptogea had the infectivity and caused the root rot to G. jamesonii, however, no distinct symptom could be observed when F. solani was inoculated alone to G. jamesonii. The co-infection test revealed that the root rot was deteriorated. The results indicated that the symptom of root rot of G. jamesonii was mainly caused by P. cryptogea, and although F. solani caused no distinct symptom on G. jamesonii, and could somewhat play accelerated and enhanced role on the symptoms caused by P. cryptogea.

关 键 词：非洲菊 根腐病 隐地疫霉 茄病镰刀菌 

分 类 号：S436.8[农业科学—农业昆虫与害虫防治]