应用Real-Time RT-PCR鉴定2个水稻品种(品系)对水稻条纹病毒的抗性差异  

Detection on Different Resistance of Two Rice Varieties Against Rice stripe virus in Real-Time RT-PCR

作　　者：杨金广[1] 方振兴[1] 张孟倩[1] 徐飞[1] 王文婷[1] 谢荔岩[1] 林奇英[1] 吴祖建[1] 谢联辉[1] 

机构地区：[1]福建省植物病毒学重点实验室,福建农林大学植物病毒研究所,福建福州350002

出　　处：《华南农业大学学报》2008年第3期25-28,共4页Journal of South China Agricultural University

基　　金：国家973项目(2006CB100203);教育部博士点专项科研基金(2004038900220050389006);国家自然科学基金(30671357);福建省教育厅科技三项费用(K03005)

摘　　要：应用Real-time RT-PCR检测了水稻条纹病毒(Rice stripe virus,RSV)在2种水稻品种(品系)武育粳3号和KT95-418的悬浮细胞内复制变化和相对含量的差异,结合传统生物学接种试验,确定了这2个品种(品系)对RSV抗性的差异.结果表明,RSV在武育粳3号的悬浮细胞内24 h达到复制高峰,病毒含量为侵染初期的7.46倍.而在KT95-418的悬浮细胞内,RSV达到复制高峰需要36 h,病毒含量为侵染初期的4.51倍.利用病毒生物学接种的方法,武育粳3号发病率达91.7%,而KT95-418仅为36.0%.由此可见,KT95-418较武育粳3号对RSV具有较高的抗病性.因此,Real-time RT-PCR方法与传统生物学接种试验方法相比,具有更高的准确性和灵敏性,可以作为传统品种抗病性鉴定的验证手段.The different resistance against Rice stripe virus （RSV） between rice Wuyujing 3 and KT95- 418 was investigated by detecting the replication cycle and relative amount of RSV in two varieties rice suspension cells by using Real-time RT-PCR assay and conventional RSV infected assay. The results showed that it needed 24 and 36 h when RSV replication reached its peak in rice suspension ceils of Wuyujing 3 and KT95-418 respectively, and at their peaks, the amount of RSV increased 7.46 and 4. 51 times respectively. The infected incidences of Wuyujing 3 and KT95-418 were 91.7% and 36. 0% by conventional RSV infected method. It was showed that KT95-418 had higher resistance against RSV than Wuyujing 3. In comparison with conventional RSV infected methods, Real-time RT-PCR assay was more accurate and sensitive, and would be used to validate the result of conventional RSV infected assay.

关 键 词：Real-Time RT-PCR 水稻 水稻条纹病毒 品种抗性 悬浮细胞 

分 类 号：S435.111.49[农业科学—农业昆虫与害虫防治]