夜蛾斯氏线虫液体培养基的优化研究  

Optimization of the Liquid Medium for Steinernema feltiae(Otio)

作　　者：王军辉[1] 王欢[2] 王志春[1] 丛斌[1] 

机构地区：[1]沈阳农业大学植物保护学院,沈阳110161 [2]沈阳农业大学生物科学技术学院,沈阳110161

出　　处：《沈阳农业大学学报》2008年第4期432-436,共5页Journal of Shenyang Agricultural University

基　　金：农业部“948”项目(2001238);沈阳农业大学青年教师科研基金资助项目(2005002)

摘　　要：为寻找一种廉价的夜蛾斯氏线虫液体培养基,应用二次正交旋转组合试验设计,对一种新的夜蛾斯氏线虫液体培养基的组分配比进行了研究,结果表明:该培养基6个组分的最佳水平编码值分别为:0,2,0,-2,-1,2,即6种培养基的组分分别为:1.0g,95:5,0.9g,0.2mL,0.2mL和40mL。应用筛选出的培养基对夜蛾斯氏线虫进行液体发酵培养,20d后夜蛾斯氏线虫的产量达到最大值51.33×103 IJs.mL-1,比在同样条件下用生化培养基培养的夜蛾斯氏线虫的产量(29.33×103 IJs.mL-1)高出42.86%。In order to reduce the production cost of Steinernema feltiae （Otio） ,the method of quadratic rotation-orthogonal regressive combination design was used to select the optimal liquid medium ingredients. The results showed that the yields of S. feltiae （Otio）could be up to the maximum when the best horizontal code value of 6 ingredients were 0,2,0,-2,-1,2 respectively,corrtsponding to 1.0g,95： 5,0.9g,0.2mL,0.2mL and 40mL. The optimal liquid medium was used to culture the S. feltiae （Otio） in fermentor. After 20 days,the yield of S. feltiae （Otio） reached 51.33×10^3 IJs·mL^-1 .which increased by 42.86% compared with the yield 29.33×10^3 IJs·mL^-1 of S. feltiae （Otio） cultured by using the biochemical liquid medium in the same condition in fermentor.

关 键 词：夜蛾斯氏线虫 液体培养基 二次正交旋转组合 

分 类 号：S476.15[农业科学—农业昆虫与害虫防治] S646.2[农业科学—植物保护]