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机构地区:[1]沈阳农业大学农学院,沈阳110161 [2]辽宁省农业科学院食用菌研究所,沈阳110161
出 处:《沈阳农业大学学报》2008年第4期451-454,共4页Journal of Shenyang Agricultural University
基 金:辽宁省财政厅项目(200204)
摘 要:以香菇菌糠为研究对象,采用碱化处理、氨化处理和复合处理方式降解菌糠中的碳水化合物,结果表明:复合处理是最佳化学处理方式,中性洗涤纤维、酸性洗涤纤维、酸性洗涤木质素和纤维素含量分别下降9.31%,6.72%,20.36%和16.15%,碱化处理次之,氨化处理最差。复合处理的菌糠通过混菌一次性固体发酵试验,确定菌糠发酵的最佳菌种组合和接种量,并采用L9(34)正交试验确定最适发酵时间、发酵温度、料水比和初始pH值。结果表明:采用康宁木霉+酿酒酵母+白地霉菌株组合,接种量为15%,最适发酵时间为72h,料水比为1∶1.2,发酵温度为32℃,初始pH值为5,样品粗蛋白含量最高,可由10.60%提高到29.49%,粗纤维含量与预处理前菌糠相比下降45%。Cultural waste of Lentinus edodes Sing was used in this experiment.The result of cultural residue of Edible fugi (CREF) under alkali, ammonia and the compound treatments showed that the compound treatment was the best one, with the decrease of NDF by 9.31%,ADF by 6.72%, ADL by 20.36% and Cellulose by 16.15%, then the alkali treatment and the ammonia treatment. For the compound treatment of the CREF,the suitable strains association and inoculation rate for fermentation of CREF were obtained. The best strains association was Trichodermakoeningii,Saccharomyces cerevisiae and Geotrichumcandidum, with inouculation rate of 15%. Under the condition of fermentation time 72 hour, temperature 32℃,the ratio of material to water 1:1.2,initial pH 5 ,the effect of fermentain was the best. The content of raw protein in CREF fermentation protein feed increased from 10.60% to 29.49%, the content of CF reduced to 45%.
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