HPLC法测定人体血浆中SMZ和TMP的浓度  

Determination of Sulfamethoxazole and Trimethoprim in Plasma by HPLC

作　　者：丁晨光[1] 郑学锋[1] 钟红玲 陈雨安[1] 

机构地区：[1]解放军大连医学高等专科学校

出　　处：《中国药事》1997年第6期408-409,共2页Chinese Pharmaceutical Affairs

摘　　要：采用反相ＨＰＬＣ法，以非那西丁为内标，用甲醇直接沉淀血浆蛋白后在ＨＰＬＣ上进样，于λ２３０ｎｍ处同时测定血浆中ＳＭＺ和ＴＭＰ的浓度，ＴＭＰ和ＳＭＺ最低检出限分别为０．１５和０．４ｎｇ，该法简便、快速、灵敏。A RPHPLC method for the determination of sulfamethoxazole(SMZ) and trimethoprim(TMP) in plasma is reproted.Phenacetin was used as an internal standard.The monitoring wavelength was 230nm.The detection limit of SMZ and TMP were 015 and 04ng,respectively.This method is simple,quick and sensitive.

关 键 词：高效液相色谱 磺胺甲恶唑 甲氧苄啶 TMP SMZ 

分 类 号：R978.2[医药卫生—药品] R927.2[医药卫生—药学]