银杏叶制剂对高氧暴露早产鼠肺组织血红素加氧酶-1表达的影响  

The effect of gingkgo injection on the expression of heme oxygenase-1 in the lungs of hyperoxia-induced preterm rats

作　　者：全裕凤[1] 郑明慈[2] 张华[1] 邓毅[1] 张国风[1] 张培林[1] 

机构地区：[1]广西桂林医学院附属医院新生儿科,541001 [2]广西桂林医学院附属医院围产医学研究室,541001

出　　处：《中国新生儿科杂志》2008年第4期219-222,共4页Chinese Journal of Neonatology

基　　金：广西科技厅科技攻关基金资助(0472002-35)

摘　　要：目的探讨血红素加氧酶-1(heme oxygenase-1,HO-1)在高氧暴露早产鼠肺组织中的表达及银杏叶制剂(舒血宁)对HO-1 mRNA及HO-1蛋白表达的影响,为舒血宁防治早产儿高氧肺损伤提供理论依据。方法3日龄早产鼠随机分为高氧组、空气组和高氧+舒血宁组3组,于实验3 d及7 d时,分别用半定量反转录-聚合酶链反应法和免疫组织化学法检测各组早产鼠肺组织HO-1 mRNA的表达水平和HO-1蛋白质表达水平。结果实验第3天,空气组HO-1 mRNA、HO-1蛋白均有微弱表达;与空气组相比,高氧组HO-1 mRNA表达明显高于空气组(P<0.01),HO-1蛋白仅在巨噬细胞表达弱阳性(P<0.05);与高氧组相比,舒血宁+高氧组HO-1 mRNA的表达明显下降(P<0.01),但仍然高于空气组(P<0.05),HO-1蛋白弱阳性表达(P>0.05)。实验第7天,各组HO-1 mRNA均未见表达。空气组HO-1蛋白极微弱或无表达,高氧组HO-1蛋白在巨噬细胞呈强阳性表达,明显高于空气组(P<0.01);舒血宁+高氧组HO-1蛋白的表达较高氧组明显下降(P<0.01),但仍然高于空气组(P<0.01)。结论HO-1可能参与了早产鼠高氧肺损伤,舒血宁能够下调高氧暴露早产鼠肺组织中HO-1的表达水平。Objective To study the expression of heme oxygenase-1 （HO-1） in the lungs of hyperoxia-induced preterm rats and the effects of gingkgo injection on the expression of HO-1 mRNA and HO-1 to provide evidence for clinical usage of gingkgo injection in prevention and treatment of hyperoxiainduced lung injury in preterm infants. Methods Three days old, Sprague-Dawley preterm rats were randomly assigned to hyperoxia group （90% oxygen）, room air group and hyperoxia ± gingkgo injection group. The rats were sacrificed after 3 days and 7 days of exposure, the expression of HO-1 mRNA and HO-1 in the lungs were measured by reverse transcription polymerase chain reaction （RT-PCR） and by immunohistochemical techniques respectively. Results On day 3, the expression of HO-1 mRNA （0. 17 ± 0. 08 ） and HO-1 （ 7.23 ± 0. 63 ） turned weakly positive of rats in room air group; the expression of HO-1 mRNA of rats in hyperoxia group was significantly higher than that of room air group （0. 72 ± 0. 33, P 〈 0. 01 ）, the expression of HO-1 was shown only on macrophages and it was significantly increased compared with rats in room air group （ 18.54 ± 6.55, P 〈 0. 05 ） ; the expression of HO-1 mRNA of rats in hyperoxia ± gingkgo injection group was significantly reduced compared with those in hyperoxia group （0. 43 ± 0. 18, P 〈 0. 01 ）, yet higher than that of room air group （ P 〈 0. 01 ） ; the expression of HO-1 in hyperoxia ＋ gingkgo injection group was not significantly reduced in hyperoxia ＋ gingkgo injection group （ 14. 85 ± 8.14, P 〉 0. 05）. On day 7, the expression of HO-1 mRNA was negative of rats in each group; the expression of HO-1 extremely weak or negative of rats in room air group, but shown strongly positive on macrophages of rats in hyperoxia group and was significantly higher than that of room air group （51.24 ± 18. 32, P 〈 0.01 ） ; the expression of HO-1 was significantly reduced of rats in hyperoxia ＋ gingkgo injection group compared with that of hyperoxia

关 键 词：血红素加氧酶-1 肺 血红素 

分 类 号：R285.5[医药卫生—中药学]