细胞间黏附分子-1在粉防己碱对抗大鼠移植心脏慢性排斥中的作用  

Effect of intercellular adhesion molecule-1 on chronic rejection of rat heart transplantation treated with tetrandrine

作　　者：徐振萍[1] 刘忠民[1] 沈文律[1] 张烨峰[1] 刘翔[1] 

机构地区：[1]广东省深圳市龙岗区人民医院,518172

出　　处：《中国实用医药》2008年第19期4-5,共2页China Practical Medicine

基　　金：深圳市科技和信息局科研基金资助(项目编号:200404194)

摘　　要：目的研究细胞间黏附分子-1(ICAM-1)在粉防己碱(TET)对抗移植心脏慢性排斥反应中的作用。方法Wistar-Wistar和SD-Wistar大鼠间的颈部心脏移植分别作为同系和异系对照。TET治疗组(SD-Wistar)大鼠每天另给予50mg/kgTET灌胃。异系对照和TET治疗组大鼠每日给予环孢素A灌胃(1.5mg/kg),同系对照组不用任何药物治疗。12周后取移植心脏做组织学检查,免疫组织化学法检测组织中ICAM-1表达,以图像分析仪计算阳性率。结果异系对照动脉管壁占血管直径的平均比值为0.64±0.20,明显高于同系对照(0.23±0.07,P<0.01)和TET治疗组(0.33±0.11,P<0.05);ICAM-1在同系对照低度表达,异系对照的表达明显增强,TET治疗组的ICAM-1表达则明显减少。结论ICAM-1在慢性排斥的发生中具有重要作用,抑制ICAM-1表达可能是TET抗慢性排斥的作用机制之一。Objective To The effect of intercellular adhesion molecule-1 （ ICAM-1 ） in chronic rejection investigated treated with tetrandrine. Methods Cervical heterotopic heart transplantation was performed from Wistar to Wistar rats（ isograft controls） ,or SD to Wistar rats （allograft）. TET-treated group rats were adminis- tered with TET at a dose of 50 mg/kg body weight for 12 weeks continuously. Allograft control and TET-treated groups were also treated with cyclosporine at a dose of 1.5 mg/kg body weight. Isograft control group rats were not treated with any drug. Histological changes were monitored after 12 weeks of operation,expression of ICAM-1 was measured by immunohistochemistry, and the percentages of positive cells were calculated with computer imaging analysis system. Results In allografts,the mean ratio of depth of vessel wall and vessel diameter was 0. 64±0. 20,much higher than that in isografts（0. 23 ±0.07,P 〈0. 01 ）and TET-treated group（0.33±0. 11 ,P 〈0.05 ）. Low-grade ICAM-1 expressed in isograft group,enhanced expression was seen in allograft group, and reduced expression in TET-treated group. Conclusion ICAM-1 plays important role in chronic rejection, and inhibiting ICAM-1 expression of might be the major mechanisms of TET attenuating the development of chronic rejection.

关 键 词：粉防己碱 慢性排斥反应 移植物动脉硬化 细胞间黏附分子-1 

分 类 号：R285.5[医药卫生—中药学]