高效液相色谱法测定田基黄中异巴西红厚壳素的含量  

HPLC determination of isojacareubin in Hypericum japonicum

作　　者：熊丽[1] 梁健[1] 陈晓辉[1] 马爱玲[1] 沈振铎[1] 毕开顺[1] 

机构地区：[1]辽宁省沈阳药科大学药学院,110016

出　　处：《中国实用医药》2008年第19期8-9,共2页China Practical Medicine

摘　　要：目的建立田基黄中异巴西红厚壳素的含量测定方法。方法采用高效液相色谱法,Hy-persil C18(250mm×4.6mm,5μm)柱,流动相为乙腈-0.04%磷酸水(47∶53,v/v),流速:1.0ml/min,检测波长:254nm,柱温:30℃。结果异巴西红厚壳素线性范围1.68～56mg/L,r=0.9997(n=6);平均回收率为101.5%(RSD=2.5%,n=9)。结论该方法简便、快速、精密度好,可为田基黄的质量控制提供依据。Objective To establish a method for the determination of isojacareubin in Hypericum japonicum. Methods The separation was performed on an Hypersil C18 column （250 mm × 4.6 mm,5μm） with the mobile phase consisting of acetonitrile-0. 04% aqueous phosphonic acid（47 ： 53 ,v/v） at the flow rate of 1.0 ml/min. The detection wavelength was set at 254 nm and the column temperature was set at 30℃. Results The calibration curve was linear over the range of 1.68 ～ 56 mg/L （ r = 0. 999 7 ）. The average recoveries were 101.5% （RSD = 2.5% , n = 9 ）. Conclusion The method is simple, accurate and reproducible, and suitable for the quality control of Hypcricum japonicum.

关 键 词：田基黄 异巴西红厚壳素 高效液相色谱法 

分 类 号：R284[医药卫生—中药学]