异钩藤碱脂质体逆转人肺腺癌细胞A549/DDP对顺铂耐药的研究  

Effect of isorhynchophylline liposomes on cisplatin resistance of human lung adenocarcinoma cell line A549/DDP

作　　者：周知午[1] 周于禄[2] 

机构地区：[1]湖南省中医药研究院,湖南长沙410005 [2]中南大学湘雅三医院,湖南长沙410013

出　　处：《湖南中医药大学学报》2008年第3期29-31,共3页Journal of Hunan University of Chinese Medicine

基　　金：湖南省教育厅科学研究项目(04C432)

摘　　要：目的研究异钩藤碱脂质体(Isorhynchophylline Liposomes,IR-L)在体外对人肺腺癌细胞系A549/DDP对顺铂(DDP)的逆转作用。方法采用MTT法检测药物细胞毒性作用及耐药细胞逆转倍数,电感耦合等离子体质谱(ICP-MS)法测定细胞内顺铂浓度。结果IR-L无细胞毒浓度组(2.0μg/mL)使A549/DDP细胞对DDP的IC50由16.81 mg/L降至3.36 mg/L;低细胞毒浓度组(7.0μg/mL)的IC50降至2.34 mg/L;两组均明显提高DDP在A549/DDP细胞内的浓度,从0.92μg/L分别提高到4.62μg/L和6.89μg/L。结论IR能部分逆转A549/DDP细胞的MDR,其作用机制可能与增加细胞内药物浓度有关。Objective To determine the reversal effect of Isorhynchophylline Liposomes （IRL） on cisplatin resistance human lung adenocarcinoma cell line A549/DDP. Methods The cytotoxic effect of IR-L and the sensitivity of cisplatin were determined by MrIT assay. The intra-cellular concentration of cisplatin was detected by Inductively Coupled Plasma Mass Spectrometry （ICP-MS）. Result The maximum non-toxic concentration group of IR-L （2.0 mg/L） increased the sensitivity of cisplatin in A549/DDP, decreased IC50 of cisplatin in A549/DDP from 16.81mg/L to 3.36 mg/L, and the hyp-cytotoxic concentration （7.0μg/mL） group＇s IC50 decreased to 2.34 mg/L; IR-L groups significantly increased intracellular concentration of cisplatin in A549/ DDP cells. Conclusion The results showed that Isorhynchophylline can reverse multidrug resis- tance （MDR） of A549/DDP cells and the mechanism might be associated with the increase of intracellular concentration of cisplatin.

关 键 词：异钩藤碱 脂质体 肺腺癌细胞 逆转 耐药性 A549 DDP 

分 类 号：R965.1[医药卫生—药理学]