Bcl-2及FasL蛋白的表达与EMS发生发展的关系  

Relation between Bcl-2/FasL gene and pathogenesis of endometriosis

作　　者：钟蕙芳[1] 林婧[1] 

机构地区：[1]上海交通大学附属国际和平妇幼保健院,200030

出　　处：《中国妇幼保健》2008年第21期3004-3006,共3页Maternal and Child Health Care of China

摘　　要：目的:研究B细胞淋巴瘤/白血病基因-2蛋白(Bcl-2)和体内Fas蛋白的天然配体(FasL)基因与子宫内膜异位症之间的关系。方法:采用免疫组化法(SP法)检测Bcl-2与FasL在正常子宫内膜(19例)、异位内膜(33例)及在位内膜(11例)中的表达。结果:①正常内膜组Bcl-2与FasL主要表达于细胞浆及细胞膜上,Bcl-2在增生期的表达显著高于分泌期(P<0.05),FasL在增生期的表达显著低于分泌期(P<0.05),即Bcl-2及FasL在正常子宫内膜细胞中的表达有明显周期性。②异位内膜组Bcl-2及FasL的表达同样位于细胞浆或细胞膜,分泌期的表达与增生期相似(P>0.05),因此在异位子宫内膜中的Bcl-2与FasL呈持续表达且丧失周期性,且二者在异位内膜中的表达显著高于正常内膜(P<0.0001)。结论:具有抗凋亡活性的子宫内膜细胞逆流至盆腹腔后,因其高水平Bcl-2及FasL表达得以逃避机体免疫清除系统的影响而在异位存活、种植,为子宫内膜异位症的发生创造了先决条件。Objective： To research whether the endometriosis is associated with Bcl -2 and FasL gene. Methods： Immanohisto-chemistry method （streptavidin - peroxidase method） was used to detect the expression of Bcl-2 and FasL gene in 19 specimens of normal endometrium tissue, 33 specimens of endometriosis tissue and eutopic endometrium from patients with endometrlosis （11 cases） . Results：①The control group ： the expression of Bcl -2 and FasL predominated in the glandular cytoplasm and/or on the plasma membrane. The expressions of Bcl - 2 in proliferative phase was higher than that in secretory phase significantly （ P 〈 0. 05）. The expressions of FasL in proliferative phase was less than that in secretory phase significantly （ P 〈 0. 05 ）. So the expressions of Bcl - 2 and FasL obviously cyclic changes throughtout the menstrual cycle in nomal endometrium tissue. ②The endometriosis group ： The expressions of Bcl - 2 and FasL also predominated in the glandular cytoplasm and/or on the plasma membrane. Both Bcl - 2 and FasL expression in proliferative phase was the same as secretory phase （P 〉 0. 05）. So Bcl - 2 and FasL expression in adenomyosis had no cyclic change and their staining had remained in the whole menstrual cycle. The expressions of Bcl - 2 and FasL in endometriosis was higher than that in normal endometrium significantly （ P 〈 0. 000 1 ） . Conclusion： The retrograding of these active anti - apoptotic endometrial cells may play a key role in the pathogenesis of endometrlosis.

关 键 词：子宫内膜异位症Bcl-2 FasL免疫组织化学细胞凋亡 

分 类 号：R711.71[医药卫生—妇产科学]