钌(Ⅱ)多吡啶配合物的合成、荧光性质及与脱氧核糖核酸DNA的作用机制研究  

Synthesis,DNA-binding and Luminescent Properties of Polypyridyl Ruthenium(Ⅱ) Complex

作　　者：王海滔[1] 胡婷婷[1] 张黔玲[1] 刘剑洪[1] 任祥忠[1] 李翠华[1] 王芳[1] 张培新[1] 

机构地区：[1]深圳大学化学与化工学院,深圳518060

出　　处：《化学学报》2008年第13期1565-1571,共7页Acta Chimica Sinica

基　　金：国家自然科学基金(No.20601019);广东省自然科学基金博士启动基金(No.04300882)资助项目

摘　　要：设计合成含多个配位中心的多吡啶配体ODCIP(3,4-二氯基苯并咪唑并[4,5-f][1,10]邻菲咯啉)及其钌(Ⅱ)多吡啶配合物[Ru(bpy)2ODCIP]2+.运用元素分析、红外光谱、核磁谱和质谱对配体及配合物进行结构表征.利用紫外吸收光谱、荧光光谱和粘度法研究了[Ru(bpy)2ODCIP]2+与DNA(脱氧核糖核酸)的作用机制、与Co2+配位后与DNA的作用机制及其荧光变化情况.结果表明[Ru(bpy)2ODCIP]2+与DNA通过部分插入模式作用,[Ru(bpy)2ODCIP]2+与Co2+配位形成的双核配合物[Ru(bpy)2(ODCIP)Co]4+也能与DNA插入结合.进一步利用稳态荧光发射光谱、荧光淬灭实验等方法研究了单核配合物[Ru(bpy)2ODCIP]2+和双核配合物[Ru(bpy)2(ODCIP)Co]4+的荧光性质.The polypyridyl ligand with multi coordination sites ODCIP （ODCIP = 3,4-dichloroimidazo[4,5-f][1,10]phenanthroline） and its ruthenium（Ⅱ） complex [Ru（bpy）2ODCIP]^2＋ were synthesized and characterized by elemental analyses, IR, NMR and FAB-MS, The DNA-binding properties of complex [Ru（bpy）2ODCIP]^2＋ were studied by absorption spectra, fluorescent spectra and viscosity methods. The coordination of [Ru（bpy）2ODCIP]^2＋ with Co^2＋ was also investigated. The results indicated that [Ru（bpy）2ODCIP]^2＋ bound to DNA by part intercalation. The binuclear complex [Ru（bpy）2（ODCIP）Co]^4＋ could be formed by [Ru（bpy）2ODCIP]^2＋ and Co^2＋, and it bound to DNA by intercalation. Furthermore, the luminescent properties of [Ru（bpy）2ODCIP]^2＋ and [Ru（bpy）2（ODCIP）Co]^4＋ were investigated by fluorescent spectra.

关 键 词：脱氧核糖核酸DNA 多吡啶钌配合物 双核 插入 荧光 

分 类 号：O641.4[理学—物理化学]