栀子提取物四对单纯疱疹病毒1型感染小鼠脑内VP16 mRNA的影响  

Effect of the Gardenia extracts T9 on VP16 mRNA in herpes simplex virus type-1 infected mice＇s brains

作　　者：王意忠[1] 时宇静[2] 黄洋[2] 郭姗姗[2] 姜晶[1] 苏丹[2] 赵晔[2] 高英杰[2] 崔晓兰[2] 

机构地区：[1]北京大学航天临床医学院,100049 [2]中国中医科学院中药研究所,北京100700

出　　处：《中华微生物学和免疫学杂志》2008年第6期515-518,共4页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金面上项目（30371754）;国家十一五科技支撑项目（2006BA108B04-02）

摘　　要：目的观察不同时间栀子提取物1、9对单纯疱疹病毒1型（HSV-1）感染后的BALB／c小鼠脑内间层蛋白16（VP16）的影响，探讨栀子提取物1、9抗HSV-1的作用机制。方法采用对HSV-1易感的BALB／c小鼠，以HSV-1 VP16为研究对象，借助RT-PCR方法检测栀子提取物1、9对小鼠脑内HSV VP16的影响。结果栀子提取物T9各给药组在同一时间内VP16 mRNA相对表达量均低于病毒对照组。结论栀子提取物1、9能够使HSV感染小鼠脑内VP16 mRNA相对表达量降低，可能是它抗HSV的作用机制之一。Objective To investigate the effect of the Gardenia extracts T9 on herpes simplex vires （HSV） viral protein 16（VP16） in HSV-1 infected mice＇s brains and its potential anti-viral mechanism. Methods HSV-1 infection was induced in BALB/c mice, the expression of HSV VP16 was detected by RTPCR on the 4th, 7th, 10th, 14th, and 21st day. Results The expression of VP16 mRNA decreased in both groups of large and low doses compared with virus group at the same time. Conclusion Gardenia extracts T9 can down-regulate the expression of VP16 mRNA in HSV infected mice＇s brains, and it may be one of its anti-HSV mechanisms.

关 键 词：栀子提取物T9 单纯疱疹病毒1型 间层蛋白16 

分 类 号：R28[医药卫生—中药学]