重组雌激素受体基因酵母法对南山茶子抗骨质疏松活性部位的研究  

Study of Antiosteoporosis Effective Parts of the seeds of C.serrdserrate by a Recombinant Yeast

作　　者：吴小娟[1] 李剑[2] 刘芸[2] 林红景[1] 冯宝民[1] 王永奇[1] 

机构地区：[1]大连大学生物工程学院药物研究所,辽宁大连116622 [2]中科院生态环境研究中心水生态毒理研究组,北京100085

出　　处：《辽宁中医杂志》2008年第7期1060-1061,共2页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30772714)

摘　　要：目的:明确南山茶子抗骨质疏松的有效部位。方法:用系统溶剂分离法将南山茶子醇提物分成极性不同的组分,利用重组人雌激素受体基因酵母生物检测法筛选山茶抗骨质疏松的有效部位。结果:5个组分都表现出类雌激素和抗雌激素的双重效应,活性强弱依次为乙酸乙酯层>醇提物>正丁醇层>石油醚层>水层。结论:乙酸乙酯部位为山茶抗骨质疏松的有效部位。Objective：To identify the antiosteoporosis effective parts of the seeds of Camellia Japonica.Method：The seeds of Camellia japonica extract was separated into fou parts was investigated by a recombinant yeast screening assay.Rr fractions according polarity,its antiosteoporosis effectiveesult：The five fractins showed estrogenic activity and antiestrogenic activity,the order of the estrogenic activity of these fractions is the ethyl acetate〉the alcohol extraction〉the normal butyl alcohol〉the petroleum ether〉the water extract.Conclusion：The ethyl acetate is the antionsteoporosis effective parts of the seeds of Camellia japonica.

关 键 词：南山茶 重组人基因酵母 骨质疏松 有效部位 

分 类 号：R285.5[医药卫生—中药学]