溃结灵对溃疡性结肠炎大鼠结肠黏膜IκB-α蛋白表达的作用  

Effect of Kuijieling Decoction on Proteins Expression of IκB-α in Colonic Mucosa of Ulcerative Colitis Model Rats

作　　者：李红[1] 杜群[2] 王汝俊[2] 王建华[2] 李燕舞[2] 巫燕莉[2] 

机构地区：[1]广州中医药大学中药学院,广东广州510006 [2]广州中医药大学脾胃研究所,广东广州510405

出　　处：《辽宁中医杂志》2008年第7期1100-1102,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30400613)

摘　　要：目的:观察溃结灵对溃疡性结肠炎(UC)大鼠结肠黏膜核转录因子-κB(NF-κB)的抑制蛋白(IκB)-α蛋白表达的作用,对其作用机制进行初步探讨。方法:采用三硝基苯磺酸(TNBS)法制作UC大鼠模型,大鼠随机分为以下6组:正常对照组、模型对照组、溃结灵低剂量组、中剂量组、高剂量组、阳性药柳氮磺胺吡啶(SASP)组。治疗10天后处死大鼠取结肠组织,采取免疫组化(IHC)法检测IκB-α的阳性细胞表达率。结果:模型组结肠黏膜IκB-α蛋白阳性细胞表达率明显高于正常对照组(P<0.01);溃结灵高剂量组结肠黏膜IκB-α蛋白阳性细胞表达率明显低于模型组(P<0.05)。结论:溃结灵使TNBS法UC大鼠模型结肠黏膜IκB-α蛋白阳性细胞率明显降低,这可能是其治疗UC作用的机理之一。Objective： To explore the changes of protein expression of IκB-α as well as the effects of Kuijieling Decoction（KD）in colonic mucosa of ulcerative colitis（UC）model rats.Methods： UC model rats were induced by TNBS.The rats were randomly divided into six groups as follows： normal control（NC） group,model control（MC） group,Kuijieling low dose（KLD） group,Kuijieling medium dose（KMD） group,Kuijieling high dose（KHD） group and SASP group.After 10-days treatment the rats were killed to get their colonic tissue.Detect positive rate of IκB-α expression by immunohistochemical（IHC）.Results：The positive rate of IκB-α in MC group was significantly higher than that in NC group（P〈0.01）.The positive rate of IκB-αin KHD group was significantly lower than that in MC group（P〈0.05）.Conclusion： IκB-α may be involved in the pathogenesis of UC,and KD could inhibit positive rate of IκB-α in colonic mucosa of UC model rats induced by TNBS.The inhibitory effects of KD on UC might be associated with the inhibition of positive rate of IκB-α.

关 键 词：溃结灵 溃疡性结肠炎 大鼠模型 IΚB-Α 

分 类 号：R285.5[医药卫生—中药学]