分子印迹聚合物固相萃取红椒粉中的苏丹红Ⅰ  

Molecularly imprinted solid phase extraction for detection of sudan I in red peppers

作　　者：徐莉[1] 刘英菊[1] 刘有芹[1] 刘岚[2] 

机构地区：[1]华南农业大学化学系,广东广州510642 [2]中山大学化学与化学工程学院,广东广州510275

出　　处：《化学研究与应用》2008年第7期812-815,共4页Chemical Research and Application

基　　金：国家自然科学基金项目(20202015)资助;华南农业大学校长基金项目(2005K04162)资助

摘　　要：本文以苏丹红Ⅰ为模板分子,以甲基丙烯酸(MAA)、4-乙烯基吡啶(4-VPy)为功能单体,制备了两种模板聚合物(MAA-MIP和4-VPy-MIP)。对4-VPy-MIP进行了Scatchard方程分析。分别以这两种模板聚合物为固相萃取材料来填充固相萃取柱,从掺有苏丹红Ⅰ的红椒粉中萃取苏丹红Ⅰ。本文优化了固相萃取条件。高效液相色谱检测表明,在合适的萃取条件下,采用填充4-VPy-MIP的固相萃取柱可以有效地从红椒粉中分离富集苏丹红Ⅰ。In this paper,two kinds of molecularly imprinted polymers （MIPs） were prepared with different fimctional monomers as methacrylic acid（MAA） and 4-vinylpyridine（4-VPy）. The polymeric material was characterized by scanning electronic microscope and Scatchard analysis. The polymeric particles were packed into 6cm glass columns to be invested to absorb selectively Sudan Ⅰ from red peppers. Under the extraction conditions, the high performance liquid chromatography （HPLC） indicated the MISPE packed with 4-Vpy-MIP possessed excellent selectivity toward the template comparing the MAA-MIP.

关 键 词：分子印迹聚合物 固相萃取 苏丹红Ⅰ 高效液相色谱 

分 类 号：O657.7[理学—分析化学]