盐酸二甲双胍的毛细管电泳法快速测定  

Rapid determination of metformin hydrochloride in metformin hydrochloride tablets by capillary electrophoresis

作　　者：翟海云[1] 吴燕红[1] 黄宝美[2] 黄庆华[1] 杨冰仪[1] 陈缵光[3] 

机构地区：[1]广东药学院药科学院,广东广州510006 [2]绵阳师范学院化学系,四川绵阳621000 [3]中山大学药学院,广东广州510089

出　　处：《化学研究与应用》2008年第7期923-926,共4页Chemical Research and Application

基　　金：广东省科学技术项目(2004B10401027);广东药学院博士启动基金

摘　　要：建立了毛细管电泳高频电导法快速测定片剂中盐酸二甲双胍的方法。考察了缓冲溶液、有机溶剂添加剂、毛细管长度以及分离电压和进样条件等因素对分离检测的影响。在最佳条件下5.0 min内即可实现盐酸二甲双胍分离检测,盐酸二甲双胍的线性范围为1.50μg/mL^130μg/mL,检出限为1.0μg/mL。该方法成功地测定了盐酸二甲双胍片剂中的盐酸二甲双胍。A rapid method for the determination of Metformin hydrochloride in Metformin hydrochloride tables by capillary electrophoresis with high frequency conductivity detection was described. The key factors such as the effect of running buffer, organic additive, length of capillary, separation voltage and inject time were discussed in detail Metformin hydrochloride could be separated and detected within 5.5 min under the optimum separation conditions. Metformin hydrochloride showed good linear response in the range of 1. 50μg/mL - 130 μg/mL, and the limit of detection was 1.0 μg/mL. The method was used for analysis Metformin hydrochloride in Metformin hydrochloride tables satisfactorily.

关 键 词：毛细管电泳 高频电导检测 非接触式电导检测 盐酸二甲双胍片 盐酸二甲双胍 

分 类 号：O657.1[理学—分析化学]