1，2-萘醌-4-磺酸钠分光光度法测定链霉素的研究  

Study on the determination of streptomycin with sodium 1,2-naphthoquinone-4-sulfonate by spectrophotometry

作　　者：高成庄[1,2] 吴云玲[1] 李全民[1] 

机构地区：[1]河南师范大学化学与环境科学学院,河南省环境污染控制重点实验室,河南新乡453007 [2]焦作师范专科学校化学系,河南焦作454000

出　　处：《化学研究与应用》2008年第7期939-942,共4页Chemical Research and Application

摘　　要：It was proposed to a new method for the determination of Streptomycin(STR) with 1,2-naphthoquinone-4-sulfonate(NQS) by Spectrophotometry in this paper.It was indicated that the molar ratio of russety product formed by the reaction between STR and NQS was 1∶2 at pH12.0,and ε was 3.0×103L/mol·cm at 470nm.Beer′s law is well obeyed in the range of STR concentrations of 2.91 μg/mL-145.7μg/mL.The detection limit(3σ/κ) was 2.7μg/mL,RSD=0.76 %and average recovery was over 99.0%.The method has been used for the determination of STR in pharmaceutical samples with satisfactory results.It was proposed to a new method for the determination of Streptomycin （ STR ） with 1, 2-naphthoquinone-4-sulfonate （NQS） by Spectrophotometry in this paper. It was indicated that the molar ratio of russety product formed by the reaction between STR and NQS was 1 ： 2 at pH12.0, and ε was 3.0×10^3L/mol·cm at 470nm. Beer＇s law is well obeyed in the range of STR concentrations of 2.91 μg/mL-145.7 μg/mL. The detection limit（3σ/κ） was 2.7μg/mL,BSD =0. 76 % and average recovery was over 99.0%. The method has been used for the determination of STR in pharmaceutical samples with satisfactory results.

关 键 词：链霉素 1 2-萘醌-4-磺酸钠 分光光度法 

分 类 号：O657.32[理学—分析化学]