猪胸膜肺炎放线杆菌间接ELISA检测方法的建立  

Establishment of Indirect ELISA for Porcine Actinobacillus pleuropneumoniae

作　　者：刘珊珊[1] 陈征[2] 刘健[3] 贺东生[3] 罗满林[3] 

机构地区：[1]莱芜市动物疫病预防与控制中心,山东莱芜271100 [2]中国兽医药品监察所,北京100081 [3]华南农业大学兽医学院国家重点学科预防兽医学家畜传染病实验室,广州510642

出　　处：《中国兽药杂志》2008年第7期1-4,共4页Chinese Journal of Veterinary Drug

基　　金：上海市科委科研项目资助;课题编号:027205026

摘　　要：用纯化的重组蛋白抗原作为ELISA包被抗原,通过对抗原包被浓度、血清稀释倍数、酶标二抗稀释倍数、抗原和血清反应时间、血清和酶标二抗反应时间、显色剂作用时间和中止液滴加量的优化,建立了检测胸膜肺炎放线杆菌抗体的间接ELISA方法。特异性实验证明该ELISA方法特异性较强。将建立的ELISA方法与IDEXX公司的标准试剂盒进行了比较,二者的符合率较高,说明建立的ELISA方法比较敏感,为ELISA检测方法的商品化奠定了基础。Using the purified protein antigen as the coating antigen of ELISA, the optimal conditions of the ELISA including the protein concentration for coating, dilution of serum and conjugate, reaction time of antigen and serum, that of serum and conjugate, reaction time of developer, quantum of stop solution were all determined. Indirect ELISA method detecting App antibody was established at last. The specific character of ELISA method was relative strong by specific test. The coincidence of the established ELISA method and the standard kit of IDEXX were relative high, which indicated that the established ELISA method was rather sensitive. This test has established a foundation for commercialization of detection method of ELISA.

关 键 词：胸膜肺炎放线杆菌 APXIV 间接ELISA 

分 类 号：S852.619[农业科学—基础兽医学]