鸡大肠埃希氏菌灭活疫苗生产工艺改进试验  

Avian E.coli Inactivated Vaccine Production Experiment that Improve Technological Process

作　　者：王瑞[1] 徐太辉 于国营 

机构地区：[1]吉林大学动物医学院,长春131000 [2]青岛易邦生物工程有限公司,山东青岛266032

出　　处：《中国兽药杂志》2008年第7期33-35,共3页Chinese Journal of Veterinary Drug

摘　　要：为解决鸡大肠埃希氏菌灭活疫苗生产时培养菌数低、使用时免疫反应重的问题，在EC24、EC30、EC45、EC50发酵培养中加入0．43％～0．53％葡萄糖，试验组和对照组相比，前者培养菌数增加了40．8％、疫苗产量增加了32％、降低了生产成本。采用高速离心方法排除了菌液中的毒素、减少了疫苗的副作用，蛋鸡免疫试验苗后产蛋率比免疫常规疫苗高8．7个百分点。按新工艺试制疫苗4批，安全、效力检验合格，其它各项指标检验均能达到国家标准。In order to solve the problems that the amount of avian E. coli was few and the immune reaction was too strong, when produced and used the inactivated vaccine, 0.43 -0.53% glucose was put in ferment culture of avian E. coli EC24 EC30 EC45 EC50. In this case, the quantity of bacteria increased 40.8%, the output of the vaccine improved 32% compared with the control group, the production cost was reduced. By making use of centrifugal method at high speed to produce the vaccine, the bacteria toxin was eliminated, the vaccine was free from side effect, and the laying rate of immunized chicken is 8.7percents higher than the chicken that used com- mon vaccine. According to this new craft, 4 batches experimental vaccine were produced, and when they were examined for safety and potency, the results were all up to standard. Other results were all up to national standard.

关 键 词：鸡大肠埃希氏菌 葡萄糖 灭活疫苗 改进 

分 类 号：S852.612[农业科学—基础兽医学]