木薯淀粉分支酶基因克隆及反义表达载体的构建  

Cloning of Cassava SBEII Gene and Constructing of Its Antisense Transformation Vectors

作　　者：余洁[1] 郭运玲[1] 郭安平[1] 孔华[1] 刘恩平[1] 贺立卡[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所,海口571101

出　　处：《热带作物学报》2008年第3期342-346,共5页Chinese Journal of Tropical Crops

基　　金：中国热带农业科学院科技基金项目(编号:RKY0724);中央级科研院所基本科研业务费项目(2006-2010)"热带能源植物遗传改良与分子育种"资助

摘　　要：利用RT-PCR方法,用木薯块根cDNA做模板克隆获得支链淀粉合成的关键酶基因SBEII,经序列分析,同源性为98.07%;以PCAMBIA1301为基础,构建了以块根特异表达启动子Sporamin驱动的SBEII基因反义结构的植物表达载体。To breed a new processing alcohol type of cassava the structure of the cassava starch has to be changed to inhibit the synthesis of cassava branching starch. A key enzyme gene,SBEII gene, in the branching starch synthesis was cloned by RT-PCR and its homology was 98.07 %;SBEII gene antisense transformation vector driven by the root tubers special expression promoters Sporamin was constructed based on the known vector PCAMBIA 1301.

关 键 词：木薯 SBEII基因 基因克隆 反义表达载体 

分 类 号：S533[农业科学—作物学]