腐烂茎线虫rDNA-ITS序列分析  

Sequence Analysis of Internal Transcribed Spacer of Ditylenchus destructor Ribosomal DNA

作　　者：刘先宝[1] 谭志琼[2] 葛建军 

机构地区：[1]中国热带农业科学院环境与植物保护研究所海南省热带农业有害生物检测监控重点实验室,海南儋州571737 [2]海南大学环境与植物保护学院,海南儋州571737 [3]中国检验检疫科学院动植物检疫研究所,北京100029

出　　处：《热带作物学报》2008年第3期385-389,共5页Chinese Journal of Tropical Crops

基　　金：国家科技支撑计划-进境有害生物检测关键技术研究项目(编号:2006BAKl0B06-2)资助

摘　　要：利用一对通用引物rDNA1/rDNA2扩增6个腐烂茎线虫供试群体的rDNA-ITS区域,获得序列长度不等的片段。Des-1、Des-2和Des-3群体扩增片断长度均为1130bp。而Des-5、Des-6和Des-7群体的扩增片段长度均为942bp,与以上群体相比,在ITS区具有一个188bp的缺失片段。序列比对后确定缺失片段位于ITS1区,而且该片段含有多个可重复小片段,这可能是造成碱基缺失的原因。根据遗传进化分析可将供试线虫分为两个大的群体。The internal transcribed spacer（ITS） of rDNA from 6 nematode（Ditylenchus destructor） populations in 3 host plants were amplified and sequenced. The amplification of the rDNA-ITS region from Des-1, Des-2 and Des-3 populations produced one single fragment of 1 130 bp, whereas the PCR products of the ITS from Des-5, Des-6 and Des-7 populations were 942 bp. The ITS section was proved by the sequence of rDNA-ITS region from the GenBank. There was an absent fragment of 188 bp in the ITS1 section of Des-5, Des-6 and Des-7 populations. In the absent section were found some small repeated fragments which might be the main reason for the absence in the fragment. The 6 nematodes can be grouped into two large populations based on the analysis of their genetic evolution.

关 键 词：腐烂茎线虫 RDNA-ITS 序列分析 

分 类 号：S432.45[农业科学—植物病理学]