极低频磁场对F0F1-ATPase活力的影响及其与F1旋转频率的关系  

Effects of extremely low frequency magnetic fields on hydrolysis of F0F1-ATPases and their relationship with turnover rates of F1

作　　者：陈传芳[1,3] 崔元波[2] 乐加昌[2] 霍小林[1] 宋涛[1] 

机构地区：[1]中国科学院电工研究所,北京100190 [2]中国科学院生物物理研究所,北京100190 [3]中国科学院研究生院,北京100190

出　　处：《中华劳动卫生职业病杂志》2008年第6期327-331,共5页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金资助项目（50377042）

摘　　要：目的研究极低频正弦磁场对F0F1-ATPases活力的影响及其机制。方法强度为0．1—0．5mT、频率为4．7～96．0Hz的磁场分别处理从光合细菌（Rhodospirillum rubrum）中提取的细菌色素体（chromatophores）外膜上的F0F1-ATPases。结果0．5mT，4．7、12．0、60．0、72．0、84．0、96．0Hz磁场对F0F1-ATPases水解起促进作用，0．5mT、24．0Hz磁场对F0F1-ATPases水解起抑制作用，差异均有统计学意义（P〈0．05）；0．3mT，4．7、24．0、60．0Hz的磁场对F0F1-ATPases仍有明显影响，但0．1mT的磁场则对F0F1-ATPases无明显影响。0．5mT，24．0、60．0Hz的磁场对二环己基碳二亚胺（DCCD）抑制的F0F1-ATPases仍分别有明显地抑制和促进酶活力的影响，差异有统计学意义（P〈0．05）。结论影响F0F1-ATPases活力的极低频磁场具有频率窗口，强度阈值在0．1～0-3mT之间；F0F1-ATPases，特别是F1是磁场作用的一个重要的位点。Objective To study the effects of extremely low frequency sinusoidal magnetic fields on hydrolysis of F0F1-ATPase and its mechanism. Methods The F0F1-ATPases which was localized on the outer surface of chromatophores were prepared from the cells of Rhodospirillum rubrum and were exposed to 0.1-0.5 mT,4.7-96.0 Hz magnetic fields. Results The hydrolysis activity of F0F1-ATPase was stimulated by 0.5 mT, 4.7,12.0,60.0,72.0,84.0 and 96.0 Hz magnetic fields respectively and inhibited by 0.5 mT, 24.0 Hz magnetic field（P〈0.05） ;0.3 mT,4.7,24.0 and 60.0 Hz magnetic fields also distinctly affected F0F1-ATPases activity respectively （P〈0.05）,whereas 0.1 mT exposure caused no significant changes on that activity. When the hydrolysis activity of the F0F1-ATPases was inactivated by its inhibitor DCCD, the 0.5 mT, 24.0 Hz magnetic field still inhibited the hydrolysis activity of the F0F1-ATPase and 0.5 mT, 60.0 Hz magnetic field also had stimulating effects（P〈0.05 ）. Conclusion The effects of magnetic fields on the hydrolysis activity of the F0F1-ATPases depend on not only magnetic frequency but also magnetic intensity. The threshold of magnetic intensity is between 0.1 mT and 0.3 mT. F0F1-ATPases, especially Fl-portion may be an end-point of magnetic fields.

关 键 词：电磁场 H(+)转运ATP合酶 细菌蛋白质类 

分 类 号：Q[生物学]