PLA2/AA信号途径与重楼皂苷诱导大鼠子宫平滑肌收缩活动的关系研究  

Relationship between PLA2/AA signaling pathway and rat myometrial contraction stimulated by steroidal saponins from Paris polyphylla

作　　者：苏佳[1] 刘泽源[1] 赵振虎[2] 善亚君[2] 马百平[2] 从玉文[2] 

机构地区：[1]军事医学科学院附属医院,北京100071 [2]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《军事医学科学院院刊》2008年第3期264-268,共5页Bulletin of the Academy of Military Medical Sciences

基　　金：国家自然科学基金资助项目(No30672640)

摘　　要：目的:探讨宫血宁活性成分——重楼总皂苷(TSSP)诱导大鼠离体子宫肌条收缩的机制,考察PLA2/AA信号途径与TSSP引起的大鼠离体子宫收缩的关系。方法:应用离体子宫张力测定法,观察不同PLA2/AA信号途径抑制剂对TSSP引起的大鼠离体子宫收缩作用的影响。结果:非特异性前列腺素合成酶抑制剂可明显抑制TSSP和米索前列醇引起的子宫收缩,提示二者可能存在相似机制。环氧酶1(COX-1)抑制剂SC560,环氧酶2(COX-2)抑制剂NS-398,EP受体抑制剂AH6809和脂氧酶(5-LO)抑制剂REV5901均可对TSSP缩宫作用产生明显抑制;磷脂酶A2(PLA2)抑制剂AACOCF3也可明显抑制TSSP的缩宫作用,提示PLA2/AA信号途径与TSSP的缩宫作用密切相关。结论:TSSP对大鼠子宫平滑肌收缩活动的调节与PLA2/AA信号途径的激活有关。Objective：To investigate the mechanism underlying rat myometrial contraction stimulated by the total steroidal saponins from paris polyphylla（TSSP） , an active ingredient of Gong Xue Ning, and to explore the relationship between PLA2/AA signaling pathway and TSSP stimulated rat myometrial contraction. Methods：Rat myometrial strips.were suspended vertically in 5 ml of organ baths containing modified Krebs＇solution. Muscle tension was recorded, different PLA2/AA signaling pathway inhibitors were added to observe the effects on rat myometrial contraction stimulated by TSSP. Results： Non specific prostaglandin synthetase inhibitor could significantly inhibit myometrial contraction indued by TSSP and misoprostol, siggesting that both of them had similar mechanism. Cyclooxygenase 1 （ COX-1 ） inhibitor SC560, cyclooxygenase 2 （COX-2） inhibitor NS-398, EP receptor inhibitor AH6809 and lipoxygenase（5-LO） inhibitor REV5901 could significantly inhibit myometrial contraction induced by TSSP. Phospholipase A2 （ PLA2 ） inhibitor AACOCF3 also could significantly inhibit myometrial contraction induced by TSSP, suggesting that PLA2/AA signaling pathway is closely related to mymetrial contrantion effect of TSSP. Conclusion ：The regulatory effect of TSSP on rat myometrial contraction is related to activation of PLA2/AA signaling pathway.

关 键 词：重楼总皂苷 子宫收缩 PLA2/AA信号途径 信号传导 

分 类 号：R285.5[医药卫生—中药学]