移植大鼠心脏局部CD134表达与急性排斥反应的相关性研究  

The relationship between acute rejection and infiltration of CD134^+ lymphocytes in heart allografts

作　　者：李敬来[1] 萧明第[2] 

机构地区：[1]北京大学深圳医院心血管外科,广东深圳518036 [2]上海交通大学医学院附属上海市第一人民医院心血管外科,上海200080

出　　处：《临床和实验医学杂志》2008年第7期17-18,共2页Journal of Clinical and Experimental Medicine

摘　　要：目的探讨大鼠浸润淋巴细胞CD134表达与移植心脏生存时间的关系。方法建立大鼠异位心脏移植模型,术中静脉应用CD45单克隆抗体(CD45mAb)及供体脾细胞。术后切取移植心脏,检测局部浸润细胞CD134表达情况。结果在正常大鼠心脏,CD134阳性细胞很少;在CD45mAb及联合应用供体脾细胞组,CD134阳性细胞较单用脾细胞组和不用药组均明显减少。结论排斥反应与浸润淋巴细胞的CD134表达呈正相关。Objective To analyze the relationship between survival of aUografts and CD134 expression of infiltrating eeUs. Methods A simplified heterotopie rat heart transplantation model was established, and CD45mAb （1 mg/kg） was intravenously injected or associated with donor splenoeytes （ 1 × 10^9 eells/100 g）. The transplanted heart harvested and the expression of CD134 in infiltrating cells was performed. Results In normal rat heart, few CD134 ^＋ cells could be found. The allografts in CD45mAb or associated donor splenoeyte groups had significantly lower CD134^ ＋ expression than those treated with splenoeytes and no therapy groups. Conclusion These findings suggest that rejection is positively correlated with CD134^ ＋ cells .

关 键 词：CD45单克隆抗体 心脏移植 CD134 

分 类 号：R654.2[医药卫生—外科学]