大肠埃希菌临床分离株aac(6)′-Ib-cr基因的检测  

Examination of aac(6′)-Ib-cr gene of clinical isolates in Eschericha coli

作　　者：张雪青[1] 陈增强[1] 陈俐丽[1] 余方友[1] 

机构地区：[1]温州医学院附属第一医院检验科,浙江温州325000

出　　处：《中国卫生检验杂志》2008年第7期1266-1268,共3页Chinese Journal of Health Laboratory Technology

基　　金：温州市科技局科研基金资助项目(Y20070079)

摘　　要：目的:了解温州地区大肠埃希菌临床株中质粒介导的喹诺酮类耐药基因aac(6)′-Ib-cr的分布、耐药情况。方法:收集温州医学院附属第一医院环丙沙星耐药的大肠埃希菌临床株60株,应用PCR方法检测其aac(6)′-Ib基因,琼脂稀释法检测菌株的耐药情况,DNA测序aac(6)′-Ib-cr基因变异体,用接合传递试验方法验证细菌质粒介导的耐药性的可转移性。结果:60株大肠埃希菌临床株检出10株aac(6)′-Ib,其扩增产物进行测序,结果表明4株菌在第223位(T→C或T→A)发生变异,均携带aac(6)′-Ib-cr基因;这4株菌接合传递试验成功,临床株对喹诺酮类的耐药性传递给了受体株。结论:温州地区aac(6)′-Ib-cr介导的喹诺酮类耐药的大肠埃希菌较普遍存在。Objective：To investigate the distribution and instance of plasmid - midiated quinolone resistance of clinical isolates in Eschericha coli in Wenzhou. Methods：60 clinical isolates resistant to ciprofloxacin in Eschericha coli were collected from the First Affiliated Hospital of Wenzhou. Aac（6′） -Ib gene was detected by PCR, antimicrobial susceptibility test was examined by agar dilution test, DNA sequencing was used for indentification of aac （6′） - Ib - cr and conjugation experiment was proved to find ways of transmission of resistance. Results： The gene of aac （ 6′ ） - Ib was present in 10 isolate of 60. DNA sequencing for the 10 isolates found 4 of them contained nucleotide change at position 223 （T→C,T→A）, meaning that they had aac （6′） - Ib - cr gene. Conjugation experiments were done in 4 isolates, and their resistance to quinolones was transimitted to the recipient isolates. Conclusion：Aac （6′） -Ib -cr gene of clinical isolates in Eschericha coli were popularly present in Wenzhou.

关 键 词：大肠埃希菌 质粒 aac(6′)-Ib-cr基因 

分 类 号：R446.1[医药卫生—诊断学]