3-氨基苯硼酸改良方法检测超广谱β-内酰胺酶  

Detection of extended spectrum β-lactamases by using 3-aminophenyl boronic acid method

作　　者：史跃杰[1] 杨玉林[1] 

机构地区：[1]河南省中医院检验科,郑州450002

出　　处：《中国卫生检验杂志》2008年第7期1271-1273,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:克服经典方法检测超广谱β-内酰胺酶(ESBLS)易受AmpCβ-内酰胺酶影响引起阳性率降低问题。方法:利用3-氨基苯硼酸能够可逆抑制AmpCβ-内酰胺酶的原理,使用苯硼酸改良方法与经典方法同时对119例菌株检测ES-BLS,并进行对比。结果:无AmpCβ-内酰胺酶存在时,改良方法与经典方法阳性检出率均为100%;有AmpCβ-内酰胺酶存在时,经典方法阳性率为60%,改良方法阳性检出率100%。结论:苯硼酸法与经典方法相比,具有更高的敏感性和特异性,是检测ESBLS的一个优秀方法。Objective：To overcome the low positive rate problem about the detection of extended spectrum β -lactamases （ESBLs） by classic method when AmpC β -Laetamases （pAmpCs） to be exist. Methods：According to Boronic Acid （BA） can inhibit the activity of pAmpCs. BA and classic methods were compared on the detection of ESBLs in 119 clinical isolates. Results：The positive rates of BA and classic methods are all 100% without pAmpCs ; and the positive rate of the classic and BA methods are 60. 0% and 100% respectively if pAmpCs exist. Conclusion：Boronic Acid assays are advanced for detecting ESBLs with their higher sensitivity and specificity than classic methods.

关 键 词：苯硼酸 AMPCΒ-内酰胺酶 超广谱Β-内酰胺酶 

分 类 号：R115[医药卫生—公共卫生与预防医学]