解痉多肽在应激胃黏膜适应性保护中的作用  

Role of the spasmolytic polypeptide in gastric mucosa adaptative cytoprotection from stress

作　　者：聂时南[1] 孙海晨[1] 钱晓明[1] 邵旦兵[1] 刘红梅[1] 李兆申[2] 

机构地区：[1]南京军区南京总医院,南京大学医学院临床学院急救医学科,210002 [2]第二军医大学长海医院消化内科,上海200433

出　　处：《中国急救医学》2008年第7期621-624,共4页Chinese Journal of Critical Care Medicine

基　　金：南京军区南京总医院面上课题(No.2005090)

摘　　要：目的研究解痉多肽(spasmolytic polypeptide,SP)在水浸束缚应激(WRS)大鼠胃黏膜的基因表达变化,探讨其在应激胃黏膜适应性保护中的作用。方法采用单次和重复水浸束缚应激制作模型,动态监测胃黏膜血流量(GMBF),大体及光镜下观察黏膜损伤程度(UI)及组织学变化,逆转录-多聚酶链反应(RT-PCR)检测研究SP基因表达变化,免疫组化染色进一步证实SP表达。结果①单次应激造成胃黏膜广泛损伤,但损伤指数在3、5、7d逐渐减小,至7d降为20.8%,GMBF逐渐恢复,至7d上升为正常94.5%,SP基因表达逐渐增强(0.50±0.12 vs 0.71±0.10,P<0.01),免疫组化染色计分为0.94±0.10 vs 1.50±0.13(P<0.01);②重复应激后胃黏膜产生适应性,胃黏膜血流量上升,损伤逐渐减轻,4次应激后,损伤指数降低为单次应激的22%,胃黏膜血流量上升为正常94.2%,并且胃腺区细胞增殖,SP基因表达增强(0.57±0.01 vs 0.97±0.03,P<0.01),免疫组织化学染色计分为1.25±0.11 vs 2.56±0.16(P<0.01)。结论SP可能参与了应激胃黏膜适应性细胞保护。Objective To determine the expression of SP （spasmolytic polypeptide） in gastric mucosal of rats undergone WRS, and to probe the role of SP in adaptive cytoprotection. Methods Wister rats were exposed to single or repeated WRS for 4h every other day for up to 6 days. Gastric mucosal blood flow （GMBF） was measured by LDF - 3 Flowmeter. The degree of the gastric mucosal lesions （UI） was evaluated grossly and histologically. The expression of SP was determined by RT - PCR and immunohistochemistry＇. Results ①WRS applied once produced numerous gastric mucosal erosions. UI gradually declined and GMBF restored on 3 ,Sd after stress. UI was reduced to 20.8% and GMBF increased up to 94.5% of normal value. The expression of SP was increased during the healing of stress - induced ulceration. The same results were observed by immunohistochemistry （ 0.94 ± 0.10 vs 1.50 ± 0.13 ,P 〈 0.01 ）. ②With repeated WRS, the adaptative cytoprotection against stress was developed. UI after four consecutive WRS was 22% of UI after one WRS. GMBF after four consecutive was 94.2% of normal value. Cell proliferation in the neck regions of gastric glands was activated. The expression of SP was increased by using RT - PCR （0.57 ± 0.01 vs 0.97 ± 0.03, P 〈 0.01 ） and immunohistochemistry （ 1.25 ± 0.11 vs 2.56 ± 0.16 ,P 〈 0.01 ）. Conclusion Increased expression of SP could play an important role in adaptation of gastric mucose developed after repeated WRS.

关 键 词：应激 胃黏膜 适应性细胞保护 解痉多肽 

分 类 号：R96[医药卫生—药理学]