低温保存神经干细胞移植促进脊髓损伤后轴突再生的实验研究  

Effects of cryopreserved neural stem cells transplantation on rat axonal regeneration after spinal cord injury

作　　者：王岩峰[1] 吕刚[2] 许卫兵[1] 金哲[1] 黄涛[1] 

机构地区：[1]中国医科大学附属第一医院骨科,辽宁沈阳110001 [2]辽宁医学院

出　　处：《中国骨伤》2008年第6期427-429,共3页China Journal of Orthopaedics and Traumatology

基　　金：教育部高校博士点专项科研基金(编号:20060159019);辽宁省自然科学基金(编号:20052096)

摘　　要：目的:观察低温保存(-70℃)的神经干细胞(NSCs)复苏后移植对大鼠脊髓损伤(SCI)后轴突再生的影响。方法:NSCs在处于对数生长期阶段冻存2周,复温后由5溴脱氧尿嘧啶核苷法(Brdu)标记,制备大鼠SCI模型。实验分为3组:NSCs移植组(A组)、DMEM填充组(B组)、正常对照组(C组)。脊髓损伤后立即进行移植干预,应用免疫组化法观察Brdu标记的移植细胞的存活及迁移情况,应用辣根过氧化物酶(HRP)逆行示踪法观察损伤处轴浆运输的重建。结果:复苏的NSCs经Brdu标记后移植到SCI区,在损伤脊髓区域可检测到标记的阳性细胞,辣根示踪技术显示细胞移植组较DMEM填充组阳性细胞明显多,组间差别有统计学意义。结论:低温保存的NSCs在移植到脊髓损伤区域后可存活,并参与脊髓损伤处轴浆通路的结构重建。Objective：To observe the effects of cryopreserved （-70 ℃ ）neural stem cells （NSCs）transplantation on the axon regeneration after the spinal cord injury （SCI） of rats. Methods：Neural stem cells were cultured from the hippocampus of the rat＇s embryo and identified by immunocytochemistry of nestin, then NSCs in logarithmic phage were cryopreserved （-70℃ ） for 2 weeks. Seven days after the operation of SCI ,the NSCs in were transplanted into the injured site immediately. Thirty six adult Wistar rats were randomly divided into three groups ：spinal cord injury treated with transplantation of NSCs to the injured site （Group A）, spinal cord injury received DMEM solution （Group B ）, control group （Group C ）. Then NSCs labeled with Brdu were detected by immunohistochemisty ,and the reconstruction of spinal cord were detected by horseradish peroxidase （HRP） staining. Results： NSCs can be detected in the spinal cord after transplantation. The number of HRP positive cells of Group A was higher than that of Group B. Conclusion：The transplantation of cryopreserved NSCs can survived in the injured site and promote the reconstruction. It may be a progress in the repairing of the SCI by cells transplantation.

关 键 词：脊髓损伤 神经干细胞 移植 轴突 再生 

分 类 号：R651.2[医药卫生—外科学] R657.3[医药卫生—临床医学]