太空诱变玉米核不育基因的微卫星标记  

Mapping on Two Maize Nuclear Male Sterile Genes by Space Mutagenesis Using SSR Markers

作　　者：李玉玲[1] 余永亮[2] 刘艳阳[1] 李学慧[1] 付家锋[1] 张中伟[1] 陈欢庆[1] 

机构地区：[1]河南农业大学农学院,河南郑州450002 [2]河南省农业科学院经济作物研究所,河南郑州450002

出　　处：《河南农业大学学报》2008年第3期245-249,254,共6页Journal of Henan Agricultural University

基　　金：河南省自然科学基金项目(0511032900)

摘　　要：利用随机交配多代的郑58可育株5280(Ms)与不育株5280(ms)杂交、昌7-2不育株8057(ms)与正常可育自交系黄C杂交,分别构建两个F2定位群体.在田间育性鉴定的128株和146株中,可育株与不育株分离分别为93株完全可育、35株完全不育和114株完全可育、32株完全不育.适合性测验表明,均符合3∶1孟德尔遗传分离比例.运用SSR分子标记技术和BSA分组方法,分别选用遍布在玉米10条染色体上的418对和386对SSR标记对两个群体进行多态性筛选,结果对应有17对和36对引物出现多态性.对两个F2定位群体进行基因型和连锁分析结果表明,郑58和昌7-2太空诱变玉米细胞核雄性不育基因分别位于第1和第4染色体上,微卫星标记phi 427913和umc 1160与郑58核不育基因连锁,遗传距离分别为23.8和19.4 cM;phi 006和umc 1109与昌7-2核不育基因连锁,遗传距离分别为18.0和26.3 cM.Two mapping populations were developed to detect the genic male sterile genes, respectively. The F2 mapping population for Zheng 58 mutant was developed by crossing the Zheng 58 sterile plant 5280 （ms） with the Zheng 58 fertile plant 5280（Ms） obtained through several generations of sub-matting. While the F2 mapping population for Chang 7 -2 mutant was obtained by crossing the Chang 7 - 2 sterile plant 8057 （ms） with a normal male fertile maize inbred line Huang C. The two F2 populations included 128 and 146 plants, respectively. For the Zheng 58 population, 35 complete sterile plants and 93 complete fertile plants were observed. For the Chang 7 -2 population, 32 complete sterile and 114 complete fertile plants were observed. The results of χ^2 tests showed that they were all accorded with the segregation ratio of 3： 1. According to the male fertility observed in the field experiments and BSA method, polymorphism analysis was conducted using 418 and 386 pairs of SSR primers for the two F2 populations, which were distributed in the 10 chromosomes of maize. Totally 17 and 36 pairs of SSR primers with polymorphism were found, respectively. These polymorphic primers were further used to detect the genotypes of each F2 plant. Linkage analysis showed that the two male sterility genes were located on chromosome 1 and 4 for Zheng 58 and Chang 7 -2 mutants, respectively. SSR markers phi 427913 and umc 1160 were linked with the male sterility gene in the Zheng 58 mutant, and their genetic distances from the male sterility gene were 23.8 and 19.4 cM. However, the male sterility gene in the Chang 7 - 2 mutant was linked with phi 006 and umc 1109, with their respective genetic distance of 18.0 and 26.3 cM.

关 键 词：玉米 突变体 细胞核雄性不育 微卫星标记 基因定位 

分 类 号：S513[农业科学—作物学]