玉米抗穗粒腐病近等基因系的选育  

Near Isogenic Lines Breeding with the Resistance Gene to Maize Ear Rot by Marker Assistant Selection

作　　者：李晶晶[1] 王瑞霞[2] 韩娅楠[1] 陈甲法[1] 孙小东[1] 刘春元[3] 丁俊强[1] 吴建宇[2] 

机构地区：[1]河南农业大学农学院,河南郑州450002 [2]河南农业大学生命科学院,河南郑州450002 [3]河南农业大学植物保护学院,河南郑州450002

出　　处：《河南农业大学学报》2008年第3期250-254,共5页Journal of Henan Agricultural University

基　　金：国家863项目(2006AA10Z1D5);河南省杰出人才创新基金(0521001600)

摘　　要：在QTL分析的基础上,采用感病自交系N 6为轮回亲本,抗病自交系BT-1为非轮回亲本,通过回交育种程序结合分子标记辅助选择,经过1次杂交,5次回交,1次自交,获得了分别含有第4染色体和第6染色体上抗病基因的近等基因系.Based on the QTL analysis against Fusarium ear rot, the near isogenic lines were developed as follows： using susceptible line N 6 and resistant line BT-1 as recurrent parent and non-recurrent parent, respectively, phenotypic selection was conducted, together with marker assistant selection for selecting the genetic background of N 6, and the resistance genes on chr. 4 and chr. 6 were also screened by SSR markers. After crossed once, backcrossed 5 times and selfed once, 20 NILs with resistance gene on chr. 4 and 21 NILs with resistance gene on chr. 6 were developed.

关 键 词：玉米穗粒腐病 近等基因系 表型选择 分子标记辅助选择 

分 类 号：S513[农业科学—作物学]