HPLC法测定小鼠骨中大黄酸浓度  

Determination of rhein in mouse bone by HPLC

作　　者：崔颖 李灵芝 朱江 

机构地区：[1]中国人民武装警察医学院药化教研室,天津300162 [2]中国人民武装警察医学院药理教研室,天津300162

出　　处：《中国新药杂志》2008年第13期1154-1156,共3页Chinese Journal of New Drugs

基　　金：天津市自然科学基金(033609811)

摘　　要：目的：建立大黄酸在小鼠后肢骨中药物浓度快速HPLC测定方法，初步考察大黄酸的趋骨性。方法：后肢骨样品经冷冻、粉碎、匀浆、去蛋白和有机溶剂提取处理后，RP-HPLC进行分析。采用Phenomenex ODS-C18（250mm×4．6mm，5μm）色谱柱；流动相为甲醇-乙酸水溶液-三乙胺（400：150：0．55，乙酸调pH5．0）；流速1．0mL·min^-1；柱温25℃；检测波长为420nm。结果：后肢骨中大黄酸的方法回收率在98％～106％，在0．04～20．00μg·g^-1范围内线性关系良好，相关系数为0．9999，日内、日间RSD均〈6％，重现性良好。结论：本法简便、准确、灵敏，适用于大黄酸在小鼠骨内的药物分析。大黄酸具有较高的趋骨性。Objective ：To establish a RP-HPLC method for the determination of rhein level in bone and observe its bone-targeted character. Methods：After refrigeration, fragmentation, precipitation protein and extraction, the concentration of rhein was analyzed by RP-HPLC. The separation was performed on a Phenomenex ODS-C18 column （250 mm × 4.6 mm,5 μm） with mobile phase of methanol-acetic acid water solution-triethylamine （400： 150： 0.55 ,pH 5.0）. The UV-deteetor was set at 420 nm. Results：The recoveries of rhein in bone were more than 98% and less than 106%. The calibration curve was linear in the range of 0.04 - 20.00 μg·g^-1 with a correlation coefficient of 0. 999 9. The RSDs of intra-day and inter-day were less than 6%. Conclusion：The method is simple, precise,accurate and can be used for studies of rhein in mouse bone. And rhein has the trend of bone affinity.

关 键 词：高效液相色谱法 大黄酸 趋骨性 

分 类 号：R927.2[医药卫生—药学] R286.7