肾康丸对糖尿病肾病大鼠肾脏TGF-β_1/Smad 7通路的影响  

Effects of Shenkangwan pills on the TGF-β_1/Smad 7 signaling pathway in diabetic nephropathy

作　　者：肖炜[1] 

机构地区：[1]南方医科大学中医药学院,广东广州510515

出　　处：《广东药学院学报》2008年第3期274-276,共3页Academic Journal of Guangdong College of Pharmacy

基　　金：广东省自然科学基金(7300233)

摘　　要：目的探讨肾康丸对糖尿病肾病(DN)大鼠肾脏TGF-β1/Smad 7信号通路的影响。方法用链脲佐菌素(STZ)腹腔注射法诱导建立大鼠糖尿病(DM)模型,模型成功后随机分为3组:模型对照组、开搏通组、肾康丸组,另设正常对照组。治疗8周后,取大鼠肾脏,采用免疫组化法观察各组大鼠肾脏组织纤连蛋白(FN)、转化生长因子-β1(TGF-β1)和Smad 7蛋白表达的情况。结果8周后,DN模型组大鼠与正常大鼠比较,肾脏组织中FN和TGF-β1蛋白表达明显增加,Smad 7蛋白表达明显减少。肾康丸和开搏通都可以减少FN和TGF-β1蛋白的表达,增加Smad 7蛋白的表达。结论肾康丸可以抑制DN大鼠肾脏中TGF-β1/Smad信号传导通路的激活,减少ECM的沉积,从而延缓DN进程。Objective To explore the effects of the Shenkangwan pills on the TGF-β1/Smad 7 signaling pathway in the kidneys of the DN rats. Methods The diabetic rat models were induced by intraperitoneal injection of streptozotocin （STZ） and randomly divided into 3 groups：model control group ; Capoten group and Shenkangwan group. Some other rats were used as normal control group. All rats were administered with corresponding drugs for 8 weeks. After the last administration, the kidneys of the rats were taken out. The protein expression of FN, TGF-β1 and Smad 7 were detected with the method of immune histochemistry. Results The renal tissue protein expressions of FN and TGF-β1 in the rats of DN model group were increased than those in the rats of normal group. While the protein expression of Smad 7 was decreased distinctly in these rats＇ kidneys. Compared with those in the rats of the DN model group, the protein expressions of FN and TGF-β1 in the rats of Shenkangwan and capoten group were decreased markedly. While the protein expression of Smad 7 was increased distinctly in these rats＇ kidneys. Conclusion Shenkangwan could restrain the activation of TGF-β1/Smad signaling pathway in the renal tissue of DN rats and reduced the synthesis and secretion of the ECM ,which would stay the course of the DN.

关 键 词：肾康丸 糖尿病肾病 TGF-Β1/SMAD信号通路 

分 类 号：R96[医药卫生—药理学]