骨骼肌特异性胰岛素样生长因子1及胰岛素双受体功能缺失所致小鼠2型糖尿病  

Type 2 diabetes in MKR transgenic mice caused by skeletal muscle-specific double deficiency of IGF-1 and insulin receptors

作　　者：喻嵘[1] 成细华[1] 胡伟[1] 魏开春[1] Guo Yu-bing Liu Jun-li 

机构地区：[1]湖南中医药大学基础医学院血管生物学实验室,长沙410007 [2]加拿大麦吉尔大学医学院内科医学系

出　　处：《中国糖尿病杂志》2008年第7期438-440,共3页Chinese Journal of Diabetes

基　　金：国家自然科学基金资助项目(30400604);湖南省教育厅重点资助项目(04A040)

摘　　要：目的观察骨骼肌胰岛素样生长因子1受体及胰岛素受体的功能缺失(MKR)转基因2型糖尿病小鼠的血糖及其生长情况。方法以MKR鼠自然交配繁殖的子代为研究对象,定时检测其血糖、体重、胰岛素。采用C57小鼠为对照。结果MKR鼠所有子代均出现长度为330bp的DNA片断,表明该MKR鼠后代能稳定遗传。新生MKR鼠自出生3周开始,血糖即显著增高,5周后血糖则稳定地维持在较高水平。其体重增长随年龄的增长而减慢,至4月龄时基本稳定。MKR鼠在2月龄时即表现出显著的高胰岛素血症及糖耐量减低,与对照组小鼠比较,差异有统计学意义(P<0.01)。MKR鼠在生长过程中无自然死亡发生。结论MKR鼠是研究2型糖尿病较好的动物模型之一。Objective To examine the circulating glucose and body weight in the transgenic MKR mouse model who expressed dominant-negative IGF-1 receptor and insulin receptor in skeletal muscle leading to systemic insulin resistance and diabetes. Methods MKR mice were genotyped by PCR analysis of tail DNA. And in these mice we examined the circulating glucose and body weight once a week from 1 to 16 weeks of age, and the circulating insulin and glucose tolerance at age of two-month-old by using C57 mice as controls. Results The descendents of MKR mice kept hereditary feature. And these mice had hyperglycaemia from 3 weeks of age, and an increasing body weight slowly （P 〈 0.01）. Twenty-fold significant hyperinsulinemia was observed in MKR mice,and they were glucose intolerant in 2-month-old male and female （P〈0. 01）. Conclusions The MKR mouse is an excellent model of type 2 diabetes.

关 键 词：糖尿病 2型 转基因技术 胰岛素样生长因子1受体 胰岛素受体 

分 类 号：R587.1[医药卫生—内分泌] R681.55[医药卫生—内科学]