检测PRRSV抗体的间接阻断ELISA方法的建立  

Development of indirect-blocking enzyme-linked immunosorbent assay for detection of antibodies against porcine reproductive and respiratory syndrome virus

作　　者：冉旭华[1] 赵喜红[1] 闻晓波[1] 倪宏波[1] 李树东[1] 周恩民[1] 

机构地区：[1]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319

出　　处：《中国预防兽医学报》2008年第7期544-547,556,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：黑龙江省农垦总局攻关项目(HNKXIV-08-03-01)

摘　　要：用大肠杆菌BL21(DE3)pLysS表达PRRSV重组N蛋白作为包被抗原,建立检测猪群PRRSV抗体的间接阻断ELISA方法,并对临床上采集的256份猪血清样本进行检测,结果66份呈阳性,用IDEXXPRRSV抗体检测试剂盒检测,检出的阳性血清为63份,两者的符合率为98.8%。Western blot检测结果58份阳性,两者的符合率为96.9%。试验结果表明,本试验所建立的间接阻断ELISA方法具有良好敏感性和特异性,可用于PRRS流行病学调查和疾病的诊断。An indirect blocking enzyme-linked immunosorbent assay （ELISA） for detection of antibodies against porcine respiratory and reproductive syndrome virus（PRRSV） was developed using a recombinant PRRSV nucleoprotein expressed in E.coli BL21 （DE3） pLysS. The assay was tested on 256 serum samples for the antibody against PRRSV and compared with IDEXX kit and Western blot assay, which showed a coincidence of 98.8 % with IDEXX PRRSV antibody kit and 96.9 % with Western blot. The indirect blocking ELISA was more sensitive and specific than 1DEXX PRRSV antibody kit and Western blot

关 键 词：猪繁殖与呼吸综合征病毒 重组N蛋白 间接阻断ELISA 流行病学调查 

分 类 号：S852.651[农业科学—基础兽医学]