小鼠扩展胰岛素钳夹术中糖代谢的变化  

The changes of glucose metabolism during expansive hyperinsulinemic-euglycemic clamp in mice

作　　者：刘建雷[1] 孙勤[1] 杨刚毅[1] 李伶[2] 李钶[1] 欧阳凌云[1] 陈渝[1] 刘华 唐毅 Gunther Boden 

机构地区：[1]重庆医科大学附属第二医院内分泌科,400010 [2]重庆医科大学附属第二医院检验系临床生化教研室,400010 [3]美国Mississippi大学医学中心 [4]美国Cencotor公司肿瘤研究室 [5]美国Temple大学医学院

出　　处：《中国糖尿病杂志》2008年第6期372-374,共3页Chinese Journal of Diabetes

基　　金：国家自然科学基金资助项目(30270631和30370671);国家教委春晖计划资助项目(2003-56);美国NIH基金资助项目(R01-DK58895,toG.B.)

摘　　要：目的建立小鼠扩展胰岛素钳夹技术并观察其体内糖代谢的变化。方法分别采用[3-3H]葡萄糖和2-脱氧-3H葡萄糖(2-DOG)作为示踪剂,建立自由状态下小鼠正糖-高胰岛素钳夹技术,并观察其体内糖代谢的动态改变。结果在钳夹稳定状态,血糖稳定在基础水平(7.0±1.3mmol/L),在高血浆胰岛素水平下,血浆FFA明显抑制(从1.45±0.15到0.45±0.08mmol/L,P<0.01)。葡萄糖输注率为46.7±4.7mg·kg-1.min-1,葡萄糖清除率为11.2±0.6mg·kg-1.min-1,肝糖产生被完全抑制,骨骼肌葡萄糖摄取率为20.9±2.5μmol.100g-1.min-1,而TG和TC浓度较钳夹前明显降低(P均<0.01)。平均血糖变异系数为5.19%,平均GIR变异系数为9%。结论采用上述示踪剂建立的小鼠扩展胰岛素钳夹技术,能准确评价小鼠机体胰岛素敏感性,同时能动态反映体内糖代谢变化。Objective To establish an euglycemic-hyperinsulinemic clamp technique in mice and evaluate the changes of glucose metabolism in vivo.Methods Hyperinsulinemic-euglycemic clamp technique with [3^-3H]glucose and 2-deoxy[3-^3H] glucose（2-DOG）as a tracer was establish in awake unstressed mice（insulin infusion rate：5mU·kg^-1·min^-1）.And the glucose metabolism was determined in vivo.Results During clamp steady-state,blood glucose was 7.0 ± 1.3 mmol/L,plasma free fatty acids（FFA）were significantly suppressed（from 1.45±0.15 to 0.45±0.08mmol/L,P〈0.01）,glucose infusion rates（GIR）was 46.7± 4.7mg·kg^-1 ·min^-1,glucose disappearance rate（GRd）was 11.2±0.6mg·kg^-1·min^-1,the glucose uptake rate in muscle（MGU）was 20.9 ± 2.5 μmol·100g^-1·min^-1,and hepatic glucose production（HGP）was completely inhibited,while triglyceride（TG）and cholesterol（TC）levels were remarkably decreased（all P〈0.01）.The average coefficients of variation in blood glucose and GIR were 5% and 9%,respectively.Conclusions Hyperinsulinemic-euglycemic clamp technique with [3-^3H]glucose and 2-deoxy[3-^3H]glucose（2-DOG）as a tracer was established in awake unstressed mice for evaluating insulin sensitivity and glucose turnover.

关 键 词：小鼠 胰岛素钳夹 糖代谢 胰岛素抵抗 

分 类 号：R587.1[医药卫生—内分泌]