成纤维细胞生长因子受体2在成年小鼠骨折愈合过程中的表达模式  

Spatial and temporal expression patterns of fibroblast growth factor receptor-2 during tibial fracture healing in adult mice

作　　者：陈波[1] 陈林[1] 尹良军[1] 苏楠[1] 陈剑[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所骨科,重庆400042

出　　处：《中华创伤骨科杂志》2008年第7期655-658,共4页Chinese Journal of Orthopaedic Trauma

基　　金：重庆市科技计划项目（CSTC.2004BA5016）;国家自然科学基金重点项目（30530410）

摘　　要：目的阐明成纤维细胞生长因子受体2（FGFR2）在成年小鼠骨折愈合过程中的表达模式。方法制备小鼠胫骨不稳定骨折模型，分别于骨折后3、5、7、10、14和21d6个时相点对小鼠的骨痂摄X线片，行HE染色及FGFR2、骨钙蛋白、Ⅱ型胶原、X型胶原的原位杂交检测，明确FGFR2在成骨细胞和软骨细胞中的表达变化。结果骨折后3～5d，FGFR2与骨钙蛋白在骨折处骨膜下成骨细胞中共表达；在骨折后7～14d，FGFR2与X型胶原在软骨痂肥大前软骨细胞和肥大软骨细胞中共表达。结论FGFR2参与了骨折愈合过程，并且可能是潜在的调节骨折愈合的靶基因。Objective To clarify the spatial and temporal expression patterns of fibroblast growth factor receptor-2 （FGFR2） during fracture healing in adult mice. Methods Models of instable tibial fracture of mice were adopted in the experiment. Expressions of FGFR2 in osteoblasts and chondrocytes of callus were detected at days 3, 5, 7, 10, 14 and 21 after fracture by means of X-ray, hematoxylin-eosin （HE） staining, in situ hybridization assay of FGFR2, osteocalcin （OC）, collagen type Ⅱ （Col Ⅱ）, and collagen type X （Col X） . Results On days 3 to 5 after fracture, FGFR2 and OC, a marker for mature osteoblasts, were expressed simultaneously in cells underlying periosteum at the fracture site. On days 7 to 14 after fracture, FGFR2 and Col X were simultaneously expressed in pre-hypertrophic chondrocytes and hypertrophic chondrocytes at the fracture site. Conclusion FGFR2 may be involved in fracture healing as one of the potential signaling molecules during fracture healing.

关 键 词：受体 成纤维细胞生长因子 骨折 原位杂交 

分 类 号：R683[医药卫生—骨科学]