宫颈鳞状细胞癌巨噬细胞移动抑制因子血管内皮生长因子和p16蛋白表达与临床病理的关系  

Expression of MIF, VEGF and p16 proteins and their correlation with clinicopathological features in cervical cancer

作　　者：李雯[1] 何勉[2] 曾建芳[2] 夏金堂[3] 陈连周[1] 程杨[4] 刘军秀[2] 

机构地区：[1]中山大学附属第一院外科实验室,广州510080 [2]中山大学附属第一院妇产科,广州510080 [3]广州医学院附属广州市第一人民医院外科 [4]广州医学院附属广州市第一人民医院妇产科

出　　处：《中华肿瘤杂志》2008年第7期511-514,共4页Chinese Journal of Oncology

基　　金：广东省自然科学基金资助项目（5001776）;广州市科技计划资助项目（200523-E0381）

摘　　要：目的研究巨噬细胞移动抑制因子（MIF）、血管内皮生长因子（VEGF）和p16蛋白的表达，探讨其与宫颈鳞癌临床病理特征的关系。方法采用免疫组织化学法及Westernblot，检测10例正常宫颈组织、18例宫颈上皮内瘤变（CIN）、31例宫颈鳞癌石蜡标本的组织芯片以及3例正常宫颈上皮、3例CIN（Ⅲ级）、6例宫颈鳞癌（Ⅰb、Ⅱb各3例）新鲜标本中MIF、p16和VEGF的表达情况。结果正常宫颈、CIN和宫颈鳞癌中，MIF表达率分别为0、72．2％和93．5％，p16蛋白表达率分别为20．0％、33．3％和71．0％，VEGF表达率分别为10．0％、44．4％和74．2％。MIF、p16蛋白和VEGF在宫颈鳞癌组的表达率及表达量均显著高于CIN组，差异有统计学意义。MIF在低分化宫颈鳞癌组（17例）中的表达率高于中、高分化组（14例，P=0．021），但与临床分期无关。VEGF在低分化、进展期鳞癌（17例）的表达率分别高于中、高分化和早期鳞癌组（14例；P=0．004，P=0．008）。p16蛋白表达与癌组织分化程度、临床分期均无关。Westernblot结果显示，宫颈鳞癌组织中，MIF、VEGF和p16蛋白表达水平均明显高于CIN和正常宫颈组织的表达。结论MIF、VEGF和p16蛋白过表达在CIN向宫颈鳞癌发生过程中均起作用，MIF高表达与癌组织分化程度有关，VEGF高表达与癌组织分化程度和临床分期均相关。Objective To investigate the expression of macrophage migration inhibitory factor（ MIF）, p16 and vascular endothclial growth factor （VEGF） proteins and their relationship with clinicopathological features in cervical cancer. Methods Tissue microarray （TMA） and immunohistochemistry were used to detect the expression of MIF, p16 and VEGF proteins in specimens of 10 normal cervical epithelial tissues, 18 cervical intraepithelial neoplasia （CINⅡ , Ⅲ ） and 31 cervical squamous cell carcinomas. Western blotting was used to detect the expression of MIF, pl6 and VEGF proteins in fresh samples of 3 normal cervical epithelial tissues, 3 CIN（Ⅲ） and 6 cervical squamous cell carcinomas（3 Ⅰb and 3 Ⅱb）. Results Positive expression rates of MIF were 0, 72.2% and 93.5% in the normal,CIN and carcinoma samples, 20.0% , 33.3% and 71.0% for pl6, and 10.0% , 44.4% and 74.2% for VEGF, respectively. The expression rates and levels of the three genes were significantly higher in cervical carcinomas than those in CIN. MIF expression was significantly higher in the cases with lower differentiation （17 cases, P = 0. 021 ） , and was positively correlated with VEGF expression （ P = 0. 0045 ）. VEGF expression rate was significantly higher in both cases of poorly differentiated carcinomas and those with stage lib carcinoma or beyond （ P = 0. 004, P = 0.008 ）. p16 expression was not found to be correlated with tumor differentiation or clinical stage. It was showed by Western blotting that the expression levels of MIF, VEGF and p16 were significantly higher in the carcinomas than those in CIN or normal tissues. Conclusion Expression of MIF, VEGF and p16 are probably involved in the process of cervical carcinogenesis. MIF expression is correlated with tumor differentiation VEGF expression is correlated with both tumor differentiation and clinical stage.

关 键 词：巨噬细胞移动抑制因子 血管内皮生长因子 P16蛋白 宫颈肿瘤 

分 类 号：R686[医药卫生—骨科学]