中西药结合对胃癌多药耐药的逆转和诱导凋亡作用  

Reversal and apoptosis induction of multidrug resistant gastric carcinoma cells by combination of modern medicine and traditional Chinese medicine

作　　者：侯培珍[1] 张娟[1] 赵永峰 侯先文[3] 王睿琦[4] 

机构地区：[1]包头医学院第一附属医院急诊科,内蒙古包头014010 [2]内蒙古包钢职工医院消化科,内蒙古包头014010 [3]包头医学院第一附属医院影像中心,内蒙古包头014010 [4]包头医学院第一附属医院中医科,内蒙古包头014010

出　　处：《第四军医大学学报》2008年第13期1181-1183,共3页Journal of the Fourth Military Medical University

基　　金：包头市卫生基金科研(2003G2136-25)

摘　　要：目的:探讨中西药结合对人胃癌多药耐药细胞SGC-7901/ADR的逆转和诱导凋亡作用.方法:以斑贞1号和/或氟尿嘧啶(5-FU)为阳性对照组,采用MTT法观察斑贞1号与5-FU,川芎嗪(TMP)联合对SGC-7901/ADR细胞的逆转及杀伤作用.采用流式细胞仪(FCM)测定各药物组细胞周期的变化.光镜和电镜观察药物联合前后SGC-7901/ADR细胞形态结构变化.结果:TMP明显提高SGC-7901/ADR细胞对斑贞1号+5-FU的敏感性,逆转倍数为10.39倍.细胞毒作用为:斑贞1号+5-FU+TMP>斑贞1号+5-FU>斑贞1号>5-FU(P<0.01).FCM分析证明3种药物联合与对照组相比可将细胞阻滞在DNA合成前期和静息期-G0/G1期(P<0.05).光镜和电镜下观察到癌细胞体积变小,胞体全面变圆皱缩及典型的凋亡形态学改变.结论:中西药结合对SGC-7901/ADR细胞有较强的逆转和诱导凋亡作用,为当前肿瘤治疗提供了新思路.AIM： To explore the effect of combination of modem medicine and traditional Chinese medicine on the reversal and apoptosis of gastric carcinoma cells SGC-7901/ADR with multidrug resistance. METHODS ： BanZhen-1 and/or Fluorouracil（5-FU） was considered as positive controls. MTT assay was used to investigate the effect of combination of BanZhen-1, 5-FUand Tetramethylpyrazine（TMP） on reversal and apoptosis of SGC-7901/ADR in vitro, which was observed by light microscopy. Flow cytometer （FCM） was employed to investigate the cell cycles. The morphological changes of SGC-7901/ADR were observed by transmissional electron microscopy. RESULTS： The sensitivity of SGC-7901/ ADR to BanZhen-1 ＋ 5-FU was enhanced by 300 mg/L TMP. The cytotoxicity of BanZhen-1 ＋ 5-FU also became stronger, with the reversal index（RI） as 10.39. The cytotoxicity effect of BanZhen- 1 ＋ 5-FU ＋ TMP was stronger than that of BanZhen-1 and/or 5-FU （P 〈 0. 01 ）. FCM revealed that, with the combitination of BanZhen-1, 5-FU and TMP, cell cycle was blocked at presynthetic phase and ambiguous phase-G0/G1 phase （ P 〈 0.05 ）. The light microscopy and transmission electron microscopy revealed that the size of cells exposed to BanZhen-1 ＋ 5-FU ＋ TMP became smaller and the cells strinked, displaying the typical features of apoptosis. CONCLUSION： The combination of modem medicine and traditional Chinese medicine has stronger reversal and apoptosis induction effect on SGC-7901/ADR cells and this may supply a new theoretical basis for the clinical treatment of gastric carcinoma.

关 键 词：中西医结合疗法 胃肿瘤 肿瘤细胞 多药耐药性 逆转 调亡 

分 类 号：R979.1[医药卫生—药品]